Identification of rcnA ( yohM ), a Nickel and Cobalt Resistance Gene in Escherichia coli
Open Access
- 15 April 2005
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (8), 2912-2916
- https://doi.org/10.1128/jb.187.8.2912-2916.2005
Abstract
We report here on the isolation and primary characterization of the yohM gene of Escherichia coli . We show that yohM encodes a membrane-bound polypeptide conferring increased nickel and cobalt resistance in E. coli. yohM was specifically induced by nickel or cobalt but not by cadmium, zinc, or copper. Mutation of yohM increased the accumulation of nickel inside the cell, whereas cells harboring yohM in multicopy displayed reduced intracellular nickel content. Our data support the hypothesis that YohM is the first described efflux system for nickel and cobalt in E. coli . We propose rcnA (resistance to cobalt and nickel) as the new denomination of yohM .Keywords
This publication has 17 references indexed in Scilit:
- Nickel uptake and utilization by microorganismsFEMS Microbiology Reviews, 2003
- Efflux-mediated heavy metal resistance in prokaryotesFEMS Microbiology Reviews, 2003
- Ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genesFEMS Microbiology Reviews, 2003
- NreB from Achromobacter xylosoxidans 31A Is a Nickel-Induced Transporter Conferring Nickel ResistanceJournal of Bacteriology, 2001
- A Gene Cluster Involved in Metal Homeostasis in the Cyanobacterium Synechocystis sp. Strain PCC 6803Journal of Bacteriology, 2000
- Nickel transport systems in microorganisms.Archiv für Mikrobiologie, 2000
- HypB protein of Bradyrhizobium japonicum is a metal-binding GTPase capable of binding 18 divalent nickel ions per dimer.Proceedings of the National Academy of Sciences of the United States of America, 1995
- The nik operon of Escherichia coli encodes a periplasmic binding‐protein‐dependent transport system for nickelMolecular Microbiology, 1993
- ′uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructionsFEMS Microbiology Letters, 1992
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985