A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.
Open Access
- 1 February 1992
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 1 (3), 160-163
- https://doi.org/10.1101/gr.1.3.160
Abstract
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.Keywords
This publication has 12 references indexed in Scilit:
- Rapid, nonradioactive detection of clonal T-cell receptor gene rearrangements in lymphoid neoplasms.Proceedings of the National Academy of Sciences of the United States of America, 1990
- Polymorphisms in the coding and noncoding regions of murinePgk-1 allelesBiochemical Genetics, 1990
- A quantitative Hpall-PCR assay to measure methylation of DNA from a small number of cellsNucleic Acids Research, 1990
- Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by aPCR aidedtranscipttitration assay (PATTY)Nucleic Acids Research, 1989
- The ligation amplification reaction (LAR)—Amplification of specific DNA sequences using sequential rounds of template-dependent ligationGenomics, 1989
- Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Direct Analysis of Single Nucleotide Variation in Human DNA and RNA Using In Situ Dot HybridizationDNA, 1989
- [31] Detection and localization of single base changes by denaturing gradient gel electrophoresisMethods in enzymology, 1987
- Discrimination among the transcripts of the allelic human β-globin genes βA, βS and βC using oligodeoxynucleotide hybridization probesGene, 1986
- The nucleotide sequence of a cDNA clone containing the entire coding region for mouse X-chromo- some-linked phosphoglycerate kinaseGene, 1986