An Azocarmine stain for Differential cell Analysis of the rat Anterior Hypophysis
- 1 January 1949
- journal article
- Published by Informa UK Limited in Stain Technology
- Vol. 24 (2), 103-107
- https://doi.org/10.3109/10520294909139589
Abstract
A method of staining is described which is especially designed to facilitate differentiation of the cell types of the rat anterior hypophysis. Fixation in Zenker-formol solution is recommended. Pre-staining of the nuclei by a short immersion in alum hematoxylin is followed by mordanting in anilin alcohol and a 45 minute period of staining in azocarmine solution at 60d`C. The counterstains, acid green and orange G, are dissolved in clove oil to avoid destaining of the azocarmine.Keywords
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