Current techniques for single-cell lysis
- 15 April 2008
- journal article
- review article
- Published by The Royal Society in Journal of The Royal Society Interface
- Vol. 5 (suppl_2), S131-8
- https://doi.org/10.1098/rsif.2008.0009.focus
Abstract
Owing to the small quantities of analytes and small volumes involved in single-cell analysis techniques, manipulation strategies must be chosen carefully. The lysis of single cells for downstream chemical analysis in capillaries and lab-on-a-chip devices can be achieved by optical, acoustic, mechanical, electrical or chemical means, each having their respective strengths and weaknesses. Selection of the most appropriate lysis method will depend on the particulars of the downstream cell lysate processing. Ultrafast lysis techniques such as the use of highly focused laser pulses or pulses of high voltage are suitable for applications requiring high temporal resolution. Other factors, such as whether the cells are adherent or in suspension and whether the proteins to be collected are desired to be native or denatured, will determine the suitability of detergent-based lysis methods. Therefore, careful selection of the proper lysis technique is essential for gathering accurate data from single cells.Keywords
This publication has 35 references indexed in Scilit:
- Biophysical Response to Pulsed Laser Microbeam-Induced Cell Lysis and Molecular DeliveryJournal of Biophotonics, 2008
- Examination of laser microbeam cell lysis in a PDMS microfluidic channel using time-resolved imagingLab on a Chip, 2008
- Coaxial Flow System for Chemical CytometryAnalytical Chemistry, 2007
- Sampling efficiency of a single‐cell capillary electrophoresis systemCytometry Part A, 2007
- Measurement of Gene Expression from Single Adherent Cells and Spheroids Collected Using Fast Electrical LysisAnalytical Chemistry, 2007
- Pulsed Laser Microbeam-Induced Cell Lysis: Time-Resolved Imaging and Analysis of Hydrodynamic EffectsBiophysical Journal, 2006
- Selective Encapsulation of Single Cells and Subcellular Organelles into Picoliter- and Femtoliter-Volume DropletsAnalytical Chemistry, 2005
- Investigation of laser-induced cell lysis using time-resolved imagingApplied Physics Letters, 2004
- A Quantitative Single-Cell Assay for Protein Kinase B Reveals Important Insights into the Biochemical Behavior of an Intracellular Substrate PeptideBiochemistry, 2004
- Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparationLab on a Chip, 2003