-D-Glucan as a Diagnostic Adjunct for Invasive Fungal Infections: Validation, Cutoff Development, and Performance in Patients with Acute Myelogenous Leukemia and Myelodysplastic Syndrome
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Open Access
- 15 July 2004
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Infectious Diseases
- Vol. 39 (2), 199-205
- https://doi.org/10.1086/421944
Abstract
The Glucatell (1→3)-β- d -glucan (BG) detection assay (Associates of Cape Cod) was studied as a diagnostic adjunct for invasive fungal infections (IFIs). On the basis of findings from a preliminary study of 30 candidemic subjects and 30 healthy adults, a serum BG level of ⩾60 pg/mL was chosen as the cutoff. Testing was performed with serial serum samples obtained from 283 subjects with acute myeloid leukemia or myelodysplastic syndrome who were receiving antifungal prophylaxis. At least 1 serum sample was positive for BG at a median of 10 days before the clinical diagnosis in 100% of subjects with a proven or probable IFI. IFIs included candidiasis, fusariosis, trichosporonosis, and aspergillosis. Absence of a positive BG finding had a 100% negative predictive value, and the specificity of the test was 90% for a single positive test result and ⩾96% for ⩾2 sequential positive results. The Glucatell serum BG detection assay is highly sensitive and specific as a diagnostic adjunct for IFI.Keywords
This publication has 22 references indexed in Scilit:
- Duplicated Binding Sites for (1→3)-β-d-Glucan in the Horseshoe Crab Coagulation Factor GPublished by Elsevier BV ,2002
- Defining Opportunistic Invasive Fungal Infections in Immunocompromised Patients with Cancer and Hematopoietic Stem Cell Transplants: An International ConsensusClinical Infectious Diseases, 2002
- Elevation of Blood (1→3)-Beta-D-Glucan Concentrations in Hemodialysis PatientsNephron, 2001
- Candidemia in Cancer Patients: A Prospective, Multicenter Surveillance Study by the Invasive Fungal Infection Group (IFIG) of the European Organization for Research and Treatment of Cancer (EORTC)Clinical Infectious Diseases, 1999
- Clinical Evaluation of Diagnostic Methods Using Plasma and/or Serum for Three Mycoses: Aspergillosis, Candidosis, and Pneumocystosis.Japanese journal of medical mycology, 1999
- Hematogenous trichosporonosis in cancer patients: report of 12 cases including 5 during prophylaxis with itraconazolSupportive Care in Cancer, 1999
- Risk Factors and Outcomes Associated With Identification of Aspergillus in Respiratory Specimens From Persons With HIV DiseaseSocial psychiatry. Sozialpsychiatrie. Psychiatrie sociale, 1998
- Aspergillus Fungemia: Report of Two Cases and ReviewClinical Infectious Diseases, 1995
- Lysis—centrifugation blood cultures in the detection of tissue-proven invasive candidiasis disseminated versus single-organ infectionDiagnostic Microbiology and Infectious Disease, 1993
- A new endotoxin sensitive factor associated with hemolymph coagulation system of horseshoe crab (Limulidae)FEBS Letters, 1980