Comparison Of Immunomodulator mRNA and Protein Expression in the Lungs ofStachybotrys chartarumSpore-Exposed Mice
- 1 August 2005
- journal article
- research article
- Published by Informa UK Limited in Journal of Toxicology and Environmental Health, Part A
- Vol. 68 (15), 1321-1335
- https://doi.org/10.1080/15287390590953572
Abstract
Stachybotrys chartarum is an important toxigenic fungus that has been associated with respiratory disease onset in animals and humans. It can be separated into macrocyclic trichothecene-producing and nonproducing chemotypes based on secondary metabolite production. However, effects of spores of the two chemotypes on lung inflammatory responses are poorly understood. In this study, real-time reverse-transcription polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA) were used to investigate time-course (1, 3, 6, 24, and 48 h postinstillation [PI]) relationships in mice intratracheally exposed to 300 spores/g body weight of a macrocyclic trichothecene-producing (JS 58-17) and a nonproducing (JS 58-06) S. chartarum isolate and of Cladosporium cladosporioides. There were marked differences in the magnitude and temporal patterns of mouse lung immune responses to intratracheal exposure to spores of these species at this spore dose. Both macrophage inflammatory protein 2 (MIP-2) and surfactant protein-D (SP-D) mRNA expression were significantly upregulated in lungs of JS 58-17-treated animals compared to that of all other treatment animals at 6 and 24 h PI. Heightened mRNA expression of these immunomodulators combined with comparatively depressed MIP-2 and tumor necrosis factor (TNF)-α protein expression suggests that the action of macrocyclic trichothecenes sequestered in 58-17 spores is involved. Interestingly, TNF-α protein expression in all spore treatment animal groups was also significantly increased over that in saline controls. Similarities in expression among all spore treatment animals suggest that chemicals other than toxic secondary metabolites, and possibly spore-sequestered 1,3-β-D-glucan, may contribute to lung pathogenesis.Keywords
This publication has 39 references indexed in Scilit:
- Acute Inflammatory Responses to Stachybotrys chartarum in the Lungs of Infant Rats: Time Course and Possible MechanismsToxicological Sciences, 2005
- Surfactant protein D in the female genital tractMolecular Human Reproduction, 2004
- Comparison of Inflammatory and Cytotoxic Lung Responses in Mice after Intratracheal Exposure to Spores of Two Different Stachybotrys chartarum StrainsToxicological Sciences, 2004
- Localization of Satratoxin-G in Stachybotrys chartarum Spores and Spore-Impacted Mouse Lung Using ImmunocytochemistryToxicologic Pathology, 2004
- Intranasal exposure to a damp building mould,Stachybotrys chartarum, induces lung inflammation in mice by satratoxin-independent mechanismsClinical and Experimental Allergy, 2003
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Impairment of Endotoxin-Induced Macrophage Inflammatory Protein 2 Gene Expression in Alveolar Macrophages in Streptozotocin-Induced Diabetes in MiceInfection and Immunity, 2000
- EFFECTS OF SATRATOXINS AND OTHER MACROCYCLIC TRICHOTHECENES ON IL-2 PRODUCTION AND VIABILITY OF EL-4 THYMOMA CELLSJournal of Toxicology and Environmental Health, Part A, 1999
- Induction of Cytokine mRNAs in Mice After Oral Exposure to the Trichothecene Vomitoxin (Deoxynivalenol): Relationship to Toxin Distribution and Protein Synthesis InhibitionToxicology and Applied Pharmacology, 1995
- Acute pulmonary toxicity of inhaledβ-1,3-glucan and endotoxinInflammation Research, 1992