Two Newly Identified Cat Allergens: The von Ebner Gland Protein Fel d 7 and the Latherin-Like Protein Fel d 8
- 17 May 2011
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 156 (2), 159-170
- https://doi.org/10.1159/000322879
Abstract
Introduction: Characterization of the complete IgE binding spectrum of cat allergens is important for the development of improved diagnosis and effective immunotherapeutics. While Fel d 1 remains unchallenged as the major cat allergen, we now report the isolation of two new allergens capable of binding similar concentrations of IgE in the allergic sera of some individuals. Materials and Methods: Cat tongue and submandibular salivary gland cDNA libraries were screened by DNA hybridisation and IgE immunoassay. The isolated DNA fragments were sub-cloned into an E. coli expression system and the IgE reactivity was examined with human cat-allergic sera using a DELFIA IgE quantitation assay. Results: Fel d 7, an 18 kDa von Ebner gland protein Can f 1 homologue, was isolated from the tongue library. Fel d 8, a 24-kDa latherin-like protein with homology to Equ c 5, was isolated from the submandibular library. The frequency of IgE binding of cat-allergic sera to recombinant Fel d 1, 7 and 8 was 60.5, 37.6 and 19.3%, respectively. Inhibition studies indicated some IgE binding cross-reactivity between Fel d 7 and dog dander extracts. Discussion: The study reports the isolation and characterization of two new cat allergens. The isolation of these allergens provides the opportunity to determine the role that IgE binding proteins other than Fel d 1 play in cat-allergic disease. For cat-allergic individuals with moderate to mild rhinoconjunctivitis these allergens may play a more important role in the manifestation of their allergic disease.Keywords
This publication has 36 references indexed in Scilit:
- Higher immunoglobulin E antibody levels to recombinant Fel d 1 in cat‐allergic children with asthma compared with rhinoconjunctivitisClinical & Experimental Allergy, 2008
- Early-life respiratory viral infections, atopic sensitization, and risk of subsequent development of persistent asthmaJournal of Allergy and Clinical Immunology, 2007
- Contribution of dust mite and cat specific IgE to total IgE: Relevance to asthma prevalenceJournal of Allergy and Clinical Immunology, 2007
- The role of indoor allergens in chronic allergic diseaseJournal of Allergy and Clinical Immunology, 2007
- The effect of Fel d 1‐derived T‐cell peptides on upper and lower airway outcome measurements in cat‐allergic subjectsAllergy, 2005
- Quantitative measurement of IgE antibodies to purified allergens using streptavidin linked to a high-capacity solid phaseJournal of Allergy and Clinical Immunology, 2005
- Fel d 4, a cat lipocalin allergenClinical & Experimental Allergy, 2004
- The Safety and Efficacy of ALLERVAX CAT in Cat Allergic PatientsClinical Immunology, 1999
- Monoclonal antibodies to the major feline allergen Feld I: I. Serologic and biologic activity of affinity-purified Feld I and of Feld I-depleted extractJournal of Allergy and Clinical Immunology, 1988
- Identification and Clinical Significance of Allergenic Molecules of Cat OriginAllergy, 1985