Glutathione peroxidase protein. Absence in selenium deficiency states and correlation with enzymatic activity.
Open Access
- 1 April 1986
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 77 (4), 1402-1404
- https://doi.org/10.1172/jci112449
Abstract
Glutathione peroxidase (GSHPx) activity is an indicator of selenium status in selenium-deficient individuals. Utilizing polyclonal monospecific antibodies to purified erythrocyte GSHPx, we were able to determine the relationship between enzymatic activity and protein content. In erythrocytes from a selenium-deficient individual who was treated with selenium, and in HL-60 cells grown in the absence of selenium and then returned to selenium-containing medium, there was a direct relationship between enzymatic activity and protein content. Thus, selenium deficiency results not only in a decrease of GSHPx activity, but also in a decrease of GSHPx protein.This publication has 20 references indexed in Scilit:
- Relationships between in vitro selenium supply, glutathione peroxidase activity, and phagocytic function in the HL-60 human myeloid cell line.Journal of Biological Chemistry, 1985
- Altered oxidative metabolism in selenium-deficient rat granulocytes.The Journal of Immunology, 1983
- Identification of a selenocysteine-specific aminoacyl transfer RNA from rat liverBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982
- Blood Selenium Levels and Glutathione-Peroxidase Activities in University and Chronic Intravenous Hyperalimentation SubjectsExperimental Biology and Medicine, 1981
- An Occidental Case of Cardiomyopathy and Selenium DeficiencyNew England Journal of Medicine, 1981
- [12] Radioimmunoassay of creatine kinase isoenzymesMethods in enzymology, 1981
- Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteineBiochemistry, 1978
- The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassayBiochemical Journal, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951