Effects of particle size and coating on toxicologic parameters, fecal elimination kinetics and tissue distribution of acutely ingested silver nanoparticles in a mouse model
Open Access
- 24 August 2015
- journal article
- research article
- Published by Taylor & Francis Ltd in Nanotoxicology
- Vol. 10 (3), 352-360
- https://doi.org/10.3109/17435390.2015.1072588
Abstract
Consumer exposure to silver nanoparticles (AgNP) via ingestion can occur due to incorporation of AgNP into products such as food containers and dietary supplements. AgNP variations in size and coating may affect toxicity, elimination kinetics or tissue distribution. Here, we directly compared acute administration of AgNP of two differing coatings and sizes to mice, using doses of 0.1, 1 and 10 mg/kg body weight/day administered by oral gavage for 3 days. The maximal dose is equivalent to 2000× the EPA oral reference dose. Silver acetate at the same doses was used as ionic silver control. We found no toxicity and no significant tissue accumulation. Additionally, no toxicity was seen when AgNP were dosed concurrently with a broad-spectrum antibiotic. Between 70.5% and 98.6% of the administered silver dose was recovered in feces and particle size and coating differences did not significantly influence fecal silver. Peak fecal silver was detected between 6- and 9-h post-administration and <0.5% of the administered dose was cumulatively detected in liver, spleen, intestines or urine at 48 h. Although particle size and coating did not affect tissue accumulation, silver was detected in liver, spleen and kidney of mice administered ionic silver at marginally higher levels than those administered AgNP, suggesting that silver ion may be more bioavailable. Our results suggest that, irrespective of particle size and coating, acute oral exposure to AgNP at doses relevant to potential human exposure is associated with predominantly fecal elimination and is not associated with accumulation in tissue or toxicity.Keywords
This publication has 48 references indexed in Scilit:
- Bioavailability of silver nanoparticles and ions: from a chemical and biochemical perspectiveJournal of The Royal Society Interface, 2013
- Silver Enhances Antibiotic Activity Against Gram-Negative BacteriaScience Translational Medicine, 2013
- Chemical Transformations of Nanosilver in Biological EnvironmentsACS Nano, 2012
- Physiological effects of nanoparticles on fish: A comparison of nanometals versus metal ionsEnvironment International, 2011
- Aggregation Kinetics of Citrate and Polyvinylpyrrolidone Coated Silver Nanoparticles in Monovalent and Divalent Electrolyte SolutionsEnvironmental Science & Technology, 2011
- Distribution of silver in rats following 28 days of repeated oral exposure to silver nanoparticles or silver acetateParticle and Fibre Toxicology, 2011
- A Pharmacological and Toxicological Profile of Silver as an Antimicrobial Agent in Medical DevicesAdvances in Pharmacological Sciences, 2010
- Subchronic oral toxicity of silver nanoparticlesParticle and Fibre Toxicology, 2010
- Reproducible Community Dynamics of the Gastrointestinal Microbiota following Antibiotic PerturbationInfection and Immunity, 2009
- Argyria secondary to ingestion of homemade silver solutionJournal of the American Academy of Dermatology, 2005