Dose-dependent effect of Lactobacillus rhamnosus on quantitative reduction of faecal rotavirus shedding in children
- 8 February 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Tropical Pediatrics
- Vol. 55 (5), 297-301
- https://doi.org/10.1093/tropej/fmp001
Abstract
Beneficial effects of probiotics in acute infectious diarrhoea in children are mainly seen in watery diarrhoea and viral gastroenteritis. Lactobacillus rhamnosus, one the most extensively studied probiotic strains, is effective in shortening courses of acute diarrhoea in children. However, the dose-dependent effect of Lactobacillus upon quantification of faecal rotavirus shedding in humans remains little known. Thus, an open-label randomized trial in 23 children with acute rotaviral gastroenteritis was undertaken by randomly allocating patients to receive one of the three regimens for 3 days: daily Lactobacillus rhamnosus 35 (Lcr35) with 0 CFU/day to six patients in the control group, 2 x 10(8) CFU/day to nine patients in the low-dose group, and 6 x 10(8) CFU/day to eight patients in the high-dose group. Faecal samples were collected before and after the 3-day regimen for measurements of rotavirus concentrations by ELISA. There was no statistically significant change in faecal rotavirus concentrations in either the control group (119.2 x 10(5) particles/ml vs. 23.7 x 10(5) particles/ml, p = 0.075) or the low-dose group (36.1 x 10(5) particles/ml vs. 73.5 x 10(5) particles/ml, p = 0.859). However, the high-dose group had a significant reduction of faecal rotavirus concentration (64.2 x 10(5) particles/ml vs. 9.0 x 10(5) particles/ml, p = 0.012). Without any exception, the faecal rotavirus concentrations of all eight patients in the high-dose Lcr35 group declined by 86% after 3 days when compared with those before Lcr35 administration. In conclusion, this is the first report to provide quantitative evidence of the dose-dependent effect of Lactobacillus rhamnosus, a minimal effective dose of 6 x 10(8) CFU for 3 days, upon the faecal rotavirus shedding in paediatric patients.Keywords
This publication has 10 references indexed in Scilit:
- Efficacy of Lactobacillus rhamnosus GG in acute watery diarrhoea of Indian children: A randomised controlled trialJournal of Paediatrics and Child Health, 2007
- Effects of Feeding an Infant Formula Containing Lactobacillus GG on the Colonization of the IntestineJournal of Clinical Gastroenterology, 2005
- Use of Probiotics in Children with Acute DiarrheaPediatric Drugs, 2005
- Seasonality and trend in blood lead levels of New York State childrenBMC Pediatrics, 2004
- Effect of probiotics on enterocyte bacterial translocation in vitroPediatric Surgery International, 2001
- Adherence of Probiotic Bacteria to Human Intestinal Mucus in Healthy Infants and during Rotavirus InfectionClinical and Diagnostic Laboratory Immunology, 2001
- Lactobacillus GG Administered in Oral Rehydration Solution to Children with Acute Diarrhea: A Multicenter European TrialJournal of Pediatric Gastroenterology and Nutrition, 2000
- Oral Bacterial Therapy Reduces the Duration of Symptoms and of Viral Excretion in Children with Mild DiarrheaJournal of Pediatric Gastroenterology and Nutrition, 1997
- Fecal recovery following oral administration of Lactobacillus Strain GG (ATCC 53103) in gelatine capsules to healthy volunteersInternational Journal of Food Microbiology, 1995
- Oral bacteriotherapy for viral gastroenteritisDigestive Diseases and Sciences, 1994