Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity
- 1 August 2006
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 132 (2), 319-331
- https://doi.org/10.1530/rep.1.00996
Abstract
The endometrium plays a central role among the reproductive tissues in the context of early embryo–maternal communication and pregnancy. This study investigated transcriptome profiles of endometrium samples from day 18 pregnant vs non-pregnant heifers to get insight into the molecular mechanisms involved in conditioning the endometrium for embryo attachment and implantation. Using a combination of subtracted cDNA libraries and cDNA array hybridisation, 109 mRNAs with at least twofold higher abundance in endometrium of pregnant animals and 70 mRNAs with higher levels in the control group were identified. Among the mRNAs with higher abundance in pregnant animals, at least 41 are already described as induced by interferons. In addition, transcript levels of many new candidate genes involved in the regulation of transcription, cell adhesion, modulation of the maternal immune system and endometrial remodelling were found to be increased. The different expression level was confirmed with real-time PCR for nine genes. Localisation of mRNA expression in the endometrium was shown byin situhybridisation forAGRN,LGALS3BP,LGALS9,USP18,PARP12andBST2. A comparison with similar studies in humans, mice, and revealed species-specific and common molecular markers of uterine receptivity.Keywords
This publication has 54 references indexed in Scilit:
- Gene expression profiling of bovine endometrium during the oestrous cycle: detection of molecular pathways involved in functional changesJournal of Molecular Endocrinology, 2005
- The PARP superfamilyBioEssays, 2004
- Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycleJournal of Molecular Endocrinology, 2004
- Localization of ISG15 and Conjugated Proteins in Bovine Endometrium Using Immunohistochemistry and Electron MicroscopyEndocrinology, 2004
- Microarray profiling of progesterone-regulated endometrial genes during the rhesus monkey secretory phaseReproductive Biology and Endocrinology, 2004
- New insights into the roles of agrinNature Reviews Molecular Cell Biology, 2003
- Regulation of Ipsilateral and Contralateral Bovine Oviduct Epithelial Cell Function in the Postovulation Period: A Transcriptomics Approach1Biology of Reproduction, 2003
- Determination of the transcript profile of human endometriumMolecular Human Reproduction, 2003
- Bovine Interferon-τ Stimulates the Janus Kinase-Signal Transducer and Activator of Transcription Pathway in Bovine Endometrial Epithelial Cells1Biology of Reproduction, 2001
- Interferon Tau: A Novel Pregnancy Recognition SignalAmerican Journal of Reproductive Immunology, 1997