Basic Neuroanatomical Methods
Open Access
- 1 September 1997
- journal article
- unit
- Published by Wiley in Current Protocols in Neuroscience
- Vol. 00 (1), 1.1.1-1.1.11
- https://doi.org/10.1002/0471142301.ns0101s23
Abstract
This unit covers some of the basic procedures that are common to a wide range of neuroanatomical protocols. Procedures are provided for the preparation of unfixed, fresh brain tissue as well as for perfusion fixation of animals resulting in fixed neural tissue. A variety of methods for sectioning brains are described, including frozen sectioning in a cryostat, frozen sectioning with a microtome, and sectioning with a vibratome. The choice of sectioning method depends on how the brain has been prepared and what histochemical method is to be used. Three post-sectioning procedures are provided: defatting of slide-mounted sections, thionin staining of the sections, and coating of slides with photographic emulsion for autoradiography. Finally, a procedure is described for subbing slides with gelatin, which is necessary in some protocols in order for the sections to adhere to the slides.Keywords
This publication has 1 reference indexed in Scilit:
- Experimental NeuroanatomyPublished by Oxford University Press (OUP) ,1992