Pseudo‐neutral‐loss scan for selective detection of phosphopeptides and N‐glycopeptides using liquid chromatography coupled with a hybrid linear ion‐trap/orbitrap mass spectrometer
- 2 September 2010
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 10 (21), 3916-3921
- https://doi.org/10.1002/pmic.201000290
Abstract
We describe a novel method, termed “pseudo-neutral-loss scan” MS, for selectively probing phosphopeptides and glycopeptides on a LTQ-Orbitrap mass spectrometer. The instrument has been programmed such that an in-source CID energy is applied to all species that eluted from LC column and were subsequently ionised in the electrospray ion source. Ion pairs that differ in the mass of a neutral loss of phosphoric acid or monosaccharide residues are automatically selected for CID MS/MS and further for multi-stage-activation MS3. Our method should prove useful for a highly selective and sensitive approach to identify phosphopeptides and glycopeptides from purified protein digestion without any further enrichment, and could potentially be of use in more complex mixture.Keywords
This publication has 15 references indexed in Scilit:
- Evaluation of Gas-Phase Rearrangement and Competing Fragmentation Reactions on Protein Phosphorylation Site Assignment Using Collision Induced Dissociation-MS/MS and MS3Analytical Chemistry, 2008
- Mechanistic Insights into the Multistage Gas-Phase Fragmentation Behavior of Phosphoserine- and Phosphothreonine-Containing PeptidesJournal of Proteome Research, 2008
- A Neutral Loss Activation Method for Improved Phosphopeptide Sequence Analysis by Quadrupole Ion Trap Mass SpectrometryAnalytical Chemistry, 2004
- Selective Detection of Glycopeptides on Ion Trap Mass SpectrometersAnalytical Chemistry, 2004
- Proteomic analysis of post-translational modificationsNature Biotechnology, 2003
- A novel precursor ion discovery method on a hybrid quadrupole orthogonal acceleration time-of-flight (Q-TOF) mass spectrometer for studying protein phosphorylationJournal of the American Society for Mass Spectrometry, 2002
- Quadrupole time‐of‐flight versus triple‐quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanningJournal of Mass Spectrometry, 2001
- Collisional fragmentation of glycopeptides by electrospray ionization LC/MS and LC/MS/MS: methods for selective detection of glycopeptides in protein digestsAnalytical Chemistry, 1993
- Separation of Glycopeptides by High Performance Liquid ChromatographyJournal of Cellular Biochemistry, 1982
- Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic haptenNature, 1981