Zinc‐dependent changes in ESR signals, NADPH oxidase and plasma membrane permeability in cotton roots
- 1 May 1988
- journal article
- Published by Wiley in Physiologia Plantarum
- Vol. 73 (1), 182-186
- https://doi.org/10.1111/j.1399-3054.1988.tb09214.x
Abstract
The effect of Zn2+ on the plasma membrane permeability and superoxide radical (O2-) formation in roots was studied with cotton (Gossypium hirsutum L. cv. Delta-pine 15/21) plants grown in nutrient solution with different Zn2+ supply. Compared to Zn-sufficient plants, the plasma membrane permeability of Zn-deficient plants was increased as indicated by a 3-, 5- and 2.5-fold increase in root cell leakage of K+, NO3- and organic carbon compounds, respectively. Resupply of Zn2+ to Zn-deficient plants for 12 h substantially decreased this leakage. The effects of Zn2+ on membrane permeability were closely correlated with the levels of O2- measured by electron spin resonance (ESR) spectroscopy in the microsomal membrane fraction and in the cytosol fraction of root cells. The amplitudes of the O2- -derived Tiron ESR signal also coincided with a O2- -generating oxidase activity which was strongly dependent on the presence of NADPH and FAD. The results suggest that Zn2+ directly affects the integrity of the plasma membrane, at least in part, by interfering with O2- generation by a membrane-bound NADPH oxidase.Keywords
This publication has 25 references indexed in Scilit:
- Mechanism of phosphorus‐induced zinc deficiency in cotton. III. Changes in physiological availability of zinc in plants Is mailPhysiologia Plantarum, 1987
- Mechanism of phosphorus‐induced zinc deficiency in cotton. I. Zinc deficiency‐enhanced uptake rate of phosphorusPhysiologia Plantarum, 1986
- Inhibitory effect of zinc(II) on free radical lipid peroxidation in erythrocyte membranesJournal of Free Radicals in Biology & Medicine, 1985
- Essential requirement of magnesium ion for optimal activity of the NADPH oxidase of guinea pig polymorphonuclear leukocytesBiochemical and Biophysical Research Communications, 1983
- The nature and localization of superoxide dismutase in fronds of Lemna gibba L. and the effect of copper and zinc deficiency on its activityPhysiologia Plantarum, 1982
- A critical physiological role of zinc in the structure and function of biomembranesLife Sciences, 1981
- Iron, Zinc, Free Radicals and Oxygen in Tissue Disorders and Cancer ControlPublished by Wiley ,1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- New aspects in the biological role of zinc: A stabilizer of macromolecules and biological membranesLife Sciences, 1973