Ultrasensitive Label-Free Detection of PNA–DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor
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- 14 February 2014
- journal article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 8 (3), 2632-2638
- https://doi.org/10.1021/nn4063424
Abstract
A reduced graphene oxide (R-GO)-based field-effect transistor (FET) biosensor used for ultrasensitive label-free detection of DNA via peptide nucleic acid (PNA)–DNA hybridization is reported. In this work, R-GO was prepared by reduction of GO with hydrazine, and the FET biosensor was fabricated by drop-casting the R-GO suspension onto the sensor surface. PNA instead of DNA as the capture probe was employed, and DNA detection was performed through PNA–DNA hybridization by the R-GO FET biosensor. The detection limit as low as 100 fM was achieved, which is 1 order of magnitude lower than that of the previously reported graphene FET DNA biosensor based on DNA–DNA hybridization. Moreover, the R-GO FET biosensor was able to distinguish the complementary DNA from one-base mismatched DNA and noncomplementary DNA. Interestingly, the fabricated DNA biosensor was found to have a regeneration capability. The developed R-GO FET DNA biosensor shows ultrasensitivity and high specificity, indicating its potential applications in disease diagnostics as a point-of-care tool.Keywords
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