At the time of soybean seed sowing in the field, a high soil moisture, low soil and air temperatures, and crasts formation may occur, which can lead to slow germination, poor seedling establishment, and in some cases to loss of seed vigor. Due to the importance and prevalence of soybean the aim of this study was to determine the quality and seed viability of different genotypes produced at three locations in Vojvodina during 2009 and 2010. Eight soybean varieties (Afrodita, Valjevka, Balkan, Novosadjanka, Ravnica, Ana, Vojvodjanka and Venera) produced in Vrbas, Senta and Indjija during 2009 and 2010 were tested. Seed germination was determined using Standard laboratory test, and vigor tests (cold test, and accelerated aging test). Studied genotypes baheved differently in different years and at different localities. Genotype Venera achieved high germination values in all applied tests in 2009, while genotype Afrodita had high values of the tested parameter when conventional laboratory test was applied, and the lowest values were recorded when vigor tests were applied. Values obtained in 2010 when all tests were applied were above the prescribed minimum. Locality of Vrbas proved to be more favorable for seed production in relation to localities of Indjija and Senta due to better rainfall distribution.