Antiserum to a Synthetic Peptide Recognizes the HTLV-III Envelope Glycoprotein
- 28 March 1986
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 231 (4745), 1556-1559
- https://doi.org/10.1126/science.3006246
Abstract
In a study performed to determine which regions of the human T-cell lymphotrophic virus type III (HTLV-III) may represent vaccine candidates to prevent the acquired immune deficiency syndrome (AIDS), a synthetic peptide corresponding to amino acid sequence 735 to 752 of the precursor envelope glycoprotein of HTLV-III was used to immunize rabbits. The resulting rabbit antiserum to the synthetic peptide specifically recognized the precursor envelope glycoprotein (gp160) of HTLV-III. Human sera positive for antibody to HTLV-III reacted with this peptide. These findings indicate that synthetic peptides can be used to induce an immune response directed against a native envelope glycoprotein epitope of HTLV-III. The data are discussed in terms of using synthetic peptides to identify antigenic determinants involved in the induction of protective immunity and possibly as vaccine candidates against the etiologic agent of AIDS.Keywords
This publication has 31 references indexed in Scilit:
- Expression in Escherichia coli of Open Reading Frame Gene Segments of HTLV-IIIScience, 1985
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985
- Molecular Characterization of Human T-Cell Leukemia (Lymphotropic) Virus Type III in the Acquired Immune Deficiency SyndromeScience, 1984
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Severe Acquired Immunodeficiency in Male Homosexuals, Manifested by Chronic Perianal Ulcerative Herpes Simplex LesionsNew England Journal of Medicine, 1981
- An Outbreak of Community-AcquiredPneumocystis cariniiPneumoniaNew England Journal of Medicine, 1981
- Pneumocystis cariniiPneumonia and Mucosal Candidiasis in Previously Healthy Homosexual MenNew England Journal of Medicine, 1981
- New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugatesBiochemistry, 1979
- Prediction of protein conformationBiochemistry, 1974
- Solid‐Phase Peptide SynthesisAdvances In Enzymology and Related Subjects Of Biochemistry, 1969