Effects of a Simulated CO2 Pneumoperitoneum Environment on the Proliferation, Apoptosis, and Metastasis of Cervical Cancer Cells In Vitro
Open Access
- 1 January 2014
- journal article
- Published by International Scientific Information, Inc. in Medical Science Monitor
- Vol. 20, 2497-2503
- https://doi.org/10.12659/msm.891179
Abstract
This study aimed to investigate the growth curve, cell colony formation, cell cycle, apoptosis, anti-anoikis, and ability of invasion, adhesion, and migration of cervical cancer cells after exposure to a model of a simulated CO2 pneumoperitoneum environment with different pressures and at different times. The cervical cancer cells were cultured in groups with 8 and 16 mmHg of 100% CO2 for 1, 2, 3, and 4 h in a model of a simulated environment of CO2 pneumoperitoneum. The cells in the control group were cultured in a standard environment. The growth curve was drawn through constant survival cell counts for 7 days, and the group with most obvious change was selected for subsequent experiments to detect cell colony formation, cell cycle apoptosis, and anti-anoikis, and the ability of invasion, adhesion, and migration. After a brief inhibition, the proliferation of cervical cancer cells was markedly increased and had no relationship with different CO2 pressures. Compared with the control group, the early apoptosis rate in the experimental group was higher, and the ability of invasion, migration, and adhesion decreased significantly. Cervical cancer cells stimulated by a CO2 pneumoperitoneum environment in vitro have an increased the ability to proliferate after a short period of inhibition and have reduced abilities of invasion, migration, and adhesion.Keywords
This publication has 18 references indexed in Scilit:
- Plumbagin induces the apoptosis of human tongue carcinoma cells through the mitochondria-mediated pathwayMedical Science Monitor Basic Research, 2013
- Apoptosis – associated genes and their role in predicting responses to neoadjuvant breast cancer treatmentMedical Science Monitor, 2012
- Higher CO2-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cellsWorld Journal of Gastroenterology, 2009
- A Comparison of Laparoscopically Assisted and Open Colectomy for Colon CancerNew England Journal of Medicine, 2004
- Impact of pneumoperitoneum on tumor growthSurgical Endoscopy, 2002
- Impact of Laparoscopic CO2-insufflation on Tumor-associated molecules in cultured colorectal cancer cellsSurgical Endoscopy, 2002
- Enhancement of port site metastasis by hyaluronic acid under CO2 pneumoperitoneum in a murine modelSurgical Endoscopy, 2001
- CO2 environment influences the growth of cultured human cancer cells dependent on insufflation pressureSurgical Endoscopy, 2001
- IN VITRO INHIBITION OF TUMOUR GROWTH IN A HELIUM‐RICH ENVIRONMENT: IMPLICATIONS FOR LAPAROSCOPIC SURGERYAnz Journal of Surgery, 1999
- Influence of delayed staging laparotomy after laparoscopic removal of ovarian masses later found malignantObstetrics & Gynecology, 1998