Two new Ophiostoma species from Protea caffra in Zambia
Open Access
- 18 June 2010
- journal article
- Published by Naturalis Biodiversity Center in Persoonia - Molecular Phylogeny and Evolution of Fungi
- Vol. 24 (1), 18-28
- https://doi.org/10.3767/003158510x490392
Abstract
The genus Ophiostoma (Ophiostomatales) has a global distribution and species are best known for their association with bark beetles (Curculionidae: Scolytinae) on conifers. An unusual assemblage of these fungi is closely associated with the African endemic plant genus Protea (Proteaceae). Protea-associated Ophiostoma species are ecologically atypical as they colonise the fruiting structures of various serotinous Protea species. Seven species have been described from this niche in South Africa. It has been speculated that novel species may be present in other African countries where these host plants also occur. This view was corroborated by recent collections of two unknown species from Protea caffra trees in Zambia. In the present study we evaluate the species delineation of these isolates using morphological comparisons with other Protea-associated species, differential growth studies and analyses of DNA sequence data for the β-tubulin and internal transcribed spacer (ITS1, 5.8S, ITS2) regions. As a result, the species O. protea-sedis sp. nov., and O. zambiensis sp. nov. are described here as new. This study brings the number of Protea-associated Ophiostoma species to nine and highlights the need for more inclusive surveys, including additional African countries and hosts, to elucidate species diversity in this uncharacteristic niche.Keywords
This publication has 14 references indexed in Scilit:
- Fungal radiation in the Cape Floristic Region: An analysis based on Gondwanamyces and OphiostomaMolecular Phylogenetics and Evolution, 2009
- Mite-Mediated Hyperphoretic Dispersal of Ophiostoma spp. from the Infructescences of South African Protea spp.Environmental Entomology, 2009
- Recent developments in the MAFFT multiple sequence alignment programBriefings in Bioinformatics, 2008
- Discovery of Fungus-Mite Mutualism in a Unique NicheEnvironmental Entomology, 2007
- A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum LikelihoodSystematic Biology, 2003
- Rhynchostomatoid fungi occurring on Proteaceae.2003
- MrBayes 3: Bayesian phylogenetic inference under mixed modelsBioinformatics, 2003
- Phylogeny of the Ophiostoma stenoceras: Sporothrix schenckii ComplexMycologia, 2003
- MODELTEST: testing the model of DNA substitution.Bioinformatics, 1998
- Two Divergent Intragenomic rDNA ITS2 Types within a Monophyletic Lineage of the FungusFusariumAre NonorthologousMolecular Phylogenetics and Evolution, 1997