Procyanidins (Condensed Tannins) in Green Cell Suspension Cultures of Douglas Fir Compared with Those in Strawberry and Avocado Leaves by Means of C18-Reversed-phase Chromatography

Abstract

The procyanidins (the most common type of proanthocyanidin or condensed tannin) from cell suspension cultures derived from cotyledons of Douglas fir (Pseudotsuga menziesii, Franco) were compared with those isolated from leaves of strawberry (Fragaria chiloensis var. ananassa, Bailey) and avocado (Persea gratissima, Gaertn.). Methanol (70% vol/vol) extracts from 100 mg fresh weight samples were analyzed by a combination of C18-reversed-phase columns with high-performance liquid chromatography and normal phase paper chromatography. (-)-Epicatechin and its oligomers were generally retarded longer on C18 columns than the corresponding units made of (+)-catechin when eluted with solvents made up of 5% acetic acid alone or mixed with methanol up to 15% (vol/vol). Douglas fir preparations contained the most complex set of procyanidins and consisted of oligomers of catechin and epicatechin, whereas strawberry and avocado contained mainly (+)-catechin and (-)-epicatechin derivatives, respectively.