Allergen Drives Class Switching to IgE in the Nasal Mucosa in Allergic Rhinitis
Open Access
- 15 April 2005
- journal article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 174 (8), 5024-5032
- https://doi.org/10.4049/jimmunol.174.8.5024
Abstract
IgE-expressing B cells are over 1000 times more frequent in the nasal B cell than the peripheral blood B cell population. We have investigated the provenance of these B cells in the nasal mucosa in allergic rhinitis. It is generally accepted that expression of activation-induced cytidine deaminase and class switch recombination (CSR) occur in lymphoid tissue, implying that IgE-committed B cells must migrate through the circulation to the nasal mucosa. Our detection of mRNA for activation-induced cytidine, multiple germline gene transcripts, and ε circle transcripts in the nasal mucosa of allergic, in contrast to nonallergic control subjects, however, indicates that local CSR occurs in allergic rhinitis. The germline gene transcripts and ε circle transcripts in grass pollen-allergic subjects are up-regulated during the season and also when biopsies from allergic subjects are incubated with the allergen ex vivo. These results demonstrate that allergen stimulates local CSR to IgE, revealing a potential target for topical therapies in allergic rhinitis.Keywords
This publication has 37 references indexed in Scilit:
- Analysis of immunoglobulin E VH transcripts in a bronchial biopsy of an asthmatic patient confirms bias towards VH5, and indicates local clonal expansion, somatic mutation and isotype switch eventsImmunology, 1999
- Rheumatoid factor isotype switch and somatic mutation variants within rheumatoid arthritis synoviumImmunology, 1999
- Dendritic Cells Induce Autoimmune Diabetes and Maintain Disease via De Novo Formation of Local Lymphoid TissueThe Journal of Experimental Medicine, 1998
- Antigen-driven clonal proliferation of B cells within the target tissue of an autoimmune disease. The salivary glands of patients with Sjögren's syndrome.JCI Insight, 1998
- Expression of IL-4, Cϵ RNA, and Iϵ RNA in the nasal mucosa of patients with seasonal rhinitis: Effect of topical corticosteroidsJournal of Allergy and Clinical Immunology, 1998
- Expression of ε germ‐line gene transcripts and mRNA for the ε heavy chain of IgE in nasal B cells and the effects of topical corticosteroidEuropean Journal of Immunology, 1997
- Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilonRI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells.JCI Insight, 1997
- Immunological MemoryAnnual Review of Immunology, 1993
- CD40 and IgE: synergism between anti-CD40 monoclonal antibody and interleukin 4 in the induction of IgE synthesis by highly purified human B cells.The Journal of Experimental Medicine, 1990
- Structure of human immunoglobulin gamma genes: implications for evolution of a gene familyCell, 1982