Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction
Top Cited Papers
Open Access
- 24 December 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 30 (3), 556-568
- https://doi.org/10.1038/emboj.2010.346
Abstract
The mitochondria and the endoplasmic reticulum (ER) are two organelles that critically contribute to apoptosis induction. While it is established that they communicate, how cell death signals are transmitted from the mitochondria to the ER is unknown. Here, we show that the mitochondrial fission protein Fission 1 homologue (Fis1) conveys an apoptosis signal from the mitochondria to the ER by interacting with Bap31 at the ER and facilitating its cleavage into the pro‐apoptotic p20Bap31. Exogenous apoptosis inducers likewise use this signalling route and induce the procession of Bap31. Moreover, we show that the recruitment of procaspase‐8 to the Fis1–Bap31 platform is an early event during apoptosis induction. The association of procaspase‐8 with the Fis1–Bap31 complex is dependent on the variant of death effector domain (vDED) in Bap31 and is required for the activation of procaspase‐8. This signalling pathway establishes a feedback loop by releasing Ca2+ from the ER that activates the mitochondria for apoptosis. Hence, the Fis1–Bap31 complex (ARCosome) that spans the mitochondria–ER interface serves as a platform to activate the initiator procaspase‐8, and thereby bridges two critical organelles for apoptosis signalling. There is a [Have you seen?][1] (February 2011) associated with this Article. [1]: http://dx.doi.org/10.1038/emboj.2010.352Keywords
This publication has 44 references indexed in Scilit:
- Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial InterfaceMolecular Cell, 2010
- An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology ScreenScience, 2009
- Mitofusin 2 tethers endoplasmic reticulum to mitochondriaNature, 2008
- Mitochondrial dynamics and apoptosisGenes & Development, 2008
- Chemical Inhibition of the Mitochondrial Division Dynamin Reveals Its Role in Bax/Bak-Dependent Mitochondrial Outer Membrane PermeabilizationDevelopmental Cell, 2008
- Requirement of biphasic calcium release from the endoplasmic reticulum for Fas-mediated apoptosisThe Journal of cell biology, 2006
- The Mitochondrial Fission Protein hFis1 Requires the Endoplasmic Reticulum Gateway to Induce ApoptosisMolecular Biology of the Cell, 2006
- BAP31 Is Involved in the Retention of Cytochrome P450 2C2 in the Endoplasmic ReticulumJournal of Biological Chemistry, 2006
- BAP31 and Its Caspase Cleavage Product Regulate Cell Surface Expression of Tetraspanins and Integrin-mediated Cell SurvivalPublished by Elsevier BV ,2005
- The Domains of Apoptosis: A Genomics PerspectiveScience's STKE, 2004