Phage passage after extended processing in small‐virus‐retentive filters
- 1 July 2007
- journal article
- research article
- Published by Wiley in Biotechnology and Applied Biochemistry
- Vol. 47 (3), 141-151
- https://doi.org/10.1042/ba20060254
Abstract
Retention of a two small phages (PhiX-174 and pp7) by direct-flow small-virus-retentive filters [Viresolve NFP (normal-flow parvovirus), Virosart CPV (canine parvovirus), Ultipor DV20 and Planova 20N] was studied using a commercial-process fluid. Phage passage occurred in each filter type, particularly when overloaded with phage. Clearances of pp7 and PhiX-174 were similar for any given filter brand, arguing that the two phages are equivalent for testing small-virus-retentive filters. The patterns of flux under constant pressure and instantaneous LRV (log reduction value) in relationship to cumulative phage load differed between brands, consistent with the current industry understanding that each brand possesses specific performance attributes. Phages are a powerful and universal tool for evaluating filter performance. Validation of filter performance with phages such as pp7 or PhiX-174 as models for small mammalian viruses represents an attractive alternative to the current practice.Keywords
This publication has 17 references indexed in Scilit:
- Normal‐flow virus filtration: detection and assessment of the endpoint in bioprocessingBiotechnology and Applied Biochemistry, 2005
- Characterization of Coliphage PR772 and Evaluation of Its Use for Virus Filter Performance TestingApplied and Environmental Microbiology, 2004
- Evaluation of a Quantitative Product-enhanced Reverse Transcriptase Assay to Monitor Retrovirus in mAb Cell-cultureBiologicals, 2002
- Comparison of Filtration Properties of Hepatitis B Virus, Hepatitis C Virus and Simian Virus 40 Using a Polyvinylidene Fluoride Membrane FilterVox Sanguinis, 1998
- A Validatible Porosimetric Technique for Verifying the Integrity of Virus-Retentive MembranesBiologicals, 1996
- The Use of a Microporous Polyvinylidene Fluoride (PVDF) Membrane Filter to Separate Contaminating Viral Particles from Biologically Important ProteinsBiologicals, 1996
- High‐Temperature Short‐Time Heat Inactivation of HIV and Other Viruses in Human Blood PlasmaVox Sanguinis, 1992
- Preliminary investigation of the phage φX174 crystal structureJournal of Molecular Biology, 1990
- Irreversible binding of phage .vphi.X174 to cell-bound lipopolysaccharide receptors and release of virus-receptor complexesBiochemistry, 1985
- The size of the bacteriophage T4 head in solution with comments about the dimension of virus particles as visualized by electron microscopyJournal of Molecular Biology, 1978