Identification of Weed Hosts of Tomato yellow leaf curl virus in Cyprus
Open Access
- 1 February 2011
- journal article
- Published by Scientific Societies in Plant Disease
- Vol. 95 (2), 120-125
- https://doi.org/10.1094/pdis-05-10-0346
Abstract
An extensive study was conducted during 2007 and 2008 in three major tomato production areas of Cyprus, where Tomato yellow leaf curl virus (TYLCV) is commonly found, to assess the incidence and prevalence of naturally infected weed species that could serve as TYLCV reservoirs. Approximately 4,000 of the most common dicotyledonous plants belonging to 122 species from 25 families were collected, identified, and tested for TYLCV presence using serological and molecular methods. The tests included a previously reported conventional polymerase chain reaction (PCR) assay and a real-time TaqMan PCR assay developed and optimized in this study. Real-time PCR was found to be the most sensitive technique, and enabled the detection of TYLCV in 461 samples of 49 different species belonging to the families Amaranthaceae, Chenopodiaceae, Compositae, Convolvulaceae, Cruciferae, Euphorbiaceae, Geraniaceae, Leguminosae, Malvaceae, Orobanchaceae, Plantaginaceae, Primulaceae, Solanaceae, Umbelliferae, and Urticaceae. The results further indicated that the host range of TYLCV in Cyprus is far more extensive than previously documented and, therefore, new management strategies are required. These should focus on the control of alternative virus hosts during the growing season and in crop-free periods.Keywords
This publication has 34 references indexed in Scilit:
- First Report of Tomato yellow leaf curl virus Infecting Common Bean (Phaseolus vulgaris) in GreecePlant Disease, 2007
- Tomato yellow leaf curl virus in the Dominican Republic: Characterization of an Infectious Clone, Virus Monitoring in Whiteflies, and Identification of Reservoir HostsPhytopathology®, 2002
- A rapid PCR method to discriminate between Tomato yellow leaf curl virus isolatesAnnals of Applied Biology, 2001
- Current Status and New Natural Hosts of Tomato yellow leaf curl virus (TYLCV) in SpainPlant Disease, 2001
- Tomato dwarf leaf curl virus, a new bipartite geminivirus associated with tomatoes and peppers in Jamaica and mixed infection with tomato yellow leaf curl virusPlant Pathology, 1999
- A worldwide survey of tomato yellow leaf curl virusesArchiv für die gesamte Virusforschung, 1997
- Tomato yellow leaf curl geminivirus: serological evaluation of an improved purification method 1EPPO Bulletin, 1995
- Cultural management of tomato yellow leaf curl disease in CyprusPlant Pathology, 1987
- Yellow leaf curl and other virus diseases of tomato in CyprusPlant Pathology, 1985
- Orchard Weeds as Hosts of Tomato Ringspot and Tobacco Ringspot VirusesPlant Disease, 1984