A stochastic model of self‐renewal and commitment to differentiation of the primitive hemopoietic stem cells in culture
- 1 December 1982
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 113 (3), 455-458
- https://doi.org/10.1002/jcp.1041130314
Abstract
We recently identified a murine hemopoietic stem cell colony which consists of undifferentiated (blast) cells and appears to be more primitive than CFU-GEMM in the stem cell hierarchy. The progenitors for the colony which we termed “stem cell colony” possess an extensive self-renewal capacity and the ability to generate many secondary multipotential hemopoietic colonies in culture. We replated a total of 68 stem cell colonies from cultures of murine spleen cells and analyzed the number of stem cell–and granulocyte(neutrophil)-erythrocyte-macrophage-megakaryocyte (GEMM) colony-forming cells in individual stem cell colonies. Of the 68 stem cell colonies, 35 contained progenitors (abbreviated as “S”-cells) for stem cell colonies. The distributions of S-cells and CFU-GEMM in individual stem cell colonies were extremely heterogeneous. Neither the frequency distributions of S-cells nor CFU-GEMM in stem cell colonies could be fitted well by Poisson distribution. Rather, the frequency distribution of the s-cells could be approximated by a geometric distribution and that of CFU-GEMM by an exponential distribution, both of which are variates of the gamma distribution. Our observations are in agreement with those on the distributions of CFU-S in individual spleen colonies and provided support for a stochastic model for stem cell self-renewal and commitment in culture. Application of the theory of the branching process to the distribution of S-cells revealed a distributional parameter “p” of 0.589 which is also in agreement with the earlier report on the p value for reproduction of CFU-S.Keywords
This publication has 11 references indexed in Scilit:
- Identification in culture of a class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential hemopoietic colonies.Proceedings of the National Academy of Sciences, 1982
- Clonal origin of murine hemopoietic colonies with apparent restriction to granuclocyte‐macrophage‐megakaryocyte (GMM) differentiationJournal of Cellular Physiology, 1982
- Self-renewal of hemopoietic stem cells during mixed colony formation in vitro.Proceedings of the National Academy of Sciences, 1981
- Colony formation in agar by adult bone marrow multipotential hemopoietic cellsJournal of Cellular Physiology, 1980
- Self‐maintenance capacity of CFU‐SJournal of Cellular Physiology, 1980
- Murine hemopoietic colonies in culture containing normoblasts, macrophages, and megakaryocytesAmerican Journal of Hematology, 1978
- Pure and mixed erythroid colony formation in vitro stimulated by spleen conditioned medium with no detectable erythropoietin.Proceedings of the National Academy of Sciences, 1977
- Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoitec cell culturesNature, 1976
- A STOCHASTIC MODEL OF STEM CELL PROLIFERATION, BASED ON THE GROWTH OF SPLEEN COLONY-FORMING CELLSProceedings of the National Academy of Sciences, 1964
- A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow CellsRadiation Research, 1961