Differential expression of miRNAs in the serum of patients with high‐risk oral lesions
Open Access
- 19 July 2012
- journal article
- research article
- Published by Wiley in Cancer Medicine
- Vol. 1 (2), 268-274
- https://doi.org/10.1002/cam4.17
Abstract
Oral cancer is one of the most commonly diagnosed cancers worldwide. Disease is often diagnosed at later stages, which is associated with a poor 5‐year survival rate and a high rate of local recurrence. MicroRNAs (miRNAs), a group of small, noncoding RNAs, can be isolated from blood serum samples and have demonstrated utility as biomarkers in multiple cancer types. The aim of this study was to examine the expression profiles of circulating miRNAs in the serum of patients with high‐risk oral lesions (HRLs; oral cancer or carcinoma in situ) and to explore their utility as potential oral cancer biomarkers. Global serum miRNA profiles were generated using quantitative PCR method from 1) patients diagnosed with HRLs and undergoing intent‐to‐cure surgical treatment (N = 30) and 2) a demographically matched, noncancer control group (N = 26). We next honed our list of serum miRNAs associated with disease by reducing the effects of interpatient variability; we compared serum miRNA profiles from samples taken both before and after tumor resections (N = 10). Based on these analyses, fifteen miRNAs were significantly upregulated and five were significantly downregulated based on presence of disease (minimum fold‐change >2 in at least 50% of samples, P < 0.05, permutation). Five of these miRNAs (miR‐16, let‐7b, miR‐338‐3p, miR‐223, and miR‐29a) yielded an area under the ROC curve (AUC) >0.8, suggesting utility as noninvasive biomarkers for detection of oral cancer or high‐grade lesions. Combining these serum miRNA profiles with other screening techniques could greatly improve the sensitivity in oral cancer detection.This publication has 26 references indexed in Scilit:
- Circulating micro‐RNA expression profiles in early stage nonsmall cell lung cancerInternational Journal of Cancer, 2011
- Global epidemiology of oral and oropharyngeal cancerOral Oncology, 2009
- Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseasesCell Research, 2008
- Circulating microRNAs as stable blood-based markers for cancer detectionProceedings of the National Academy of Sciences of the United States of America, 2008
- Mature miR-184 as Potential Oncogenic microRNA of Squamous Cell Carcinoma of TongueClinical Cancer Research, 2008
- Exploration of Tumor-Suppressive MicroRNAs Silenced by DNA Hypermethylation in Oral CancerCancer Research, 2008
- ROCR: visualizing classifier performance in RBioinformatics, 2005
- MicroRNA expression profiles classify human cancersNature, 2005
- RAS Is Regulated by the let-7 MicroRNA FamilyCell, 2005
- MicroRNAs: Genomics, Biogenesis, Mechanism, and FunctionCell, 2004