Detection of MGMT, RASSF1A, p15INK4B, and p14ARF promoter methylation in circulating tumor-derived DNA of central nervous system cancer patients
Open Access
- 10 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Journal of Applied Genetics
- Vol. 54 (3), 335-344
- https://doi.org/10.1007/s13353-013-0149-x
Abstract
Despite the growing understanding of the mechanisms of carcinogenesis, cancers of the central nervous system are usually associated with unfavorable prognosis. The use of an appropriate molecular marker may improve the treatment outcome by allowing early diagnosis and treatment susceptibility monitoring. Since methylation of tumor-derived DNA can be detected in the serum of cancer patients, this makes DNA methylation-based biomarkers one of the most promising diagnostic strategies. In this study, the methylation profiles of MGMT, RASSF1A, p15INK4B, and p14ARF genes were evaluated in serum free-circulating DNA and the corresponding tumor tissue in a group of 33 primary or metastatic central nervous system cancer patients. Gene promoter methylation was assessed using methylation-specific polymerase chain reaction (PCR). All the tested genes were found to be methylated to a different extent in both serum and tumor samples. In comparison to metastatic brain tumor patients, the patients with glial tumors were characterized by a higher frequency of gene hypermethylation. The hypermethylation of RASSF1A differentiated primary from metastatic brain cancers. Moreover, the gene methylation profiles observed in serum, in most cases, matched the methylation profiles detected in paired tumor samples.Keywords
This publication has 44 references indexed in Scilit:
- Conditional probability of long-term survival in glioblastomaCancer, 2012
- MGMT promoter methylation in malignant gliomasTargeted Oncology, 2010
- p14ARF promoter region methylation as a marker for gliomas diagnosisMedical Oncology, 2010
- P16 PROMOTER METHYLATION IN THE SERUM AS A BASIS FOR THE MOLECULAR DIAGNOSIS OF GLIOMASNeurosurgery, 2009
- Circulating nucleic acids (CNAs) and cancer—A surveyBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2007
- Higher Amount of Free Circulating DNA in Serum than in Plasma Is Not Mainly Caused by Contaminated Extraneous DNA during SeparationAnnals of the New York Academy of Sciences, 2006
- The tumor suppressor RASSF1A in human carcinogenesis: an update2005
- Hypermethylation of the RASSF1A gene in gliomasClinica Chimica Acta; International Journal of Clinical Chemistry, 2004
- Promoter methylation status of multiple genes in brain metastases of solid tumors.International Journal of Molecular Medicine, 2004
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences of the United States of America, 1996