Role of Cell Cycle-regulated Expression in the Localized Incorporation of Cell Wall Proteins in Yeast
Open Access
- 1 July 2006
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 17 (7), 3267-3280
- https://doi.org/10.1091/mbc.e05-08-0738
Abstract
The yeast cell wall is an essential organelle that protects the cell from mechanical damage and antimicrobial peptides, participates in cell recognition and adhesion, and is important for the generation and maintenance of normal cell shape. We studied the localization of three covalently bound cell wall proteins in Saccharomyces cerevisiae. Tip1p was found only in mother cells, whereas Cwp2p was incorporated in small-to-medium–sized buds. When the promoter regions of TIP1 and CWP2 (responsible for transcription in early G1and S/G2phases, respectively) were exchanged, the localization patterns of Tip1p and Cwp2p were reversed, indicating that the localization of cell wall proteins can be completely determined by the timing of transcription during the cell cycle. The third protein, Cwp1p, was incorporated into the birth scar, where it remained for several generations. However, we could not detect any role of Cwp1p in strengthening the birth scar wall or any functional interaction with the proteins that mark the birth scar pole as a potential future budding site. Promoter-exchange experiments showed that expression in S/G2phase is necessary but not sufficient for the normal localization of Cwp1p. Studies of mutants in which septum formation is perturbed indicate that the normal asymmetric localization of Cwp1p also depends on the normal timing of septum formation, composition of the septum, or both.Keywords
This publication has 107 references indexed in Scilit:
- Global analysis of protein localization in budding yeastNature, 2003
- Yeast Cbk1 and Mob2 Activate Daughter-Specific Genetic Programs to Induce Asymmetric Cell FatesCell, 2001
- Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structureJournal of Molecular Biology, 1999
- ASH1 mRNA localization in yeast involves multiple secondary structural elementsand Ash1 protein translationCurrent Biology, 1999
- Asymmetric Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother CellsCell, 1996
- Mother Cell–Specific HO Expression in Budding Yeast Depends on the Unconventional Myosin Myo4p and Other Cytoplasmic ProteinsCell, 1996
- Thiamine-repressible expression vectors pREP and pRIP for fission yeastGene, 1993
- α-Agglutinin expression in Saccharomyces cerevisiaeBiochemical and Biophysical Research Communications, 1989
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Demonstration of yeast scars by fluorescence microscopyExperimental Cell Research, 1963