HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres
- 6 January 2010
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (4), 1349-1354
- https://doi.org/10.1073/pnas.0913709107
Abstract
The human histone H3 variant, CENP-A, replaces the conventional histone H3 in centromeric chromatin and, together with centromerespecific DNA-binding factors, directs the assembly of the kinetochore. We purified the prenucelosomal e-CENP-A complex. We found that HJURP, a member of the complex, was required for cell cycle specific targeting of CENP-A to centromeres. HJURP facilitated efficient deposition of CENP-A/H4 tetramers to naked DNA in vitro. Bacterially expressed HJURP binds at a stoichiometric ratio to the CENP-A/H4 tetramer but not to the H3/H4 tetramer. The binding occurred through a conserved HJURP short N-terminal domain, termed CBD. The novel characteristic identified in vertebrates that we named TLTY box of CBD, was essential for formation of the HJURP-CENP-A/H4 complex. Our data identified HJURP as a vertebrate CENP-A chaperone and dissected its mode of interactions with CENP-A.Keywords
This publication has 42 references indexed in Scilit:
- HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at CentromeresCell, 2009
- Centromere-Specific Assembly of CENP-A Nucleosomes Is Mediated by HJURPCell, 2009
- Fission Yeast Scm3: A CENP-A Receptor Required for Integrity of Subkinetochore ChromatinMolecular Cell, 2009
- Fission Yeast Scm3 Mediates Stable Assembly of Cnp1/CENP-A into Centromeric ChromatinMolecular Cell, 2009
- Structural specificity in coiled-coil interactionsCurrent Opinion in Structural Biology, 2008
- Structural Basis for the Recognition of Histone H4 by the Histone-Chaperone RbAp46Structure, 2008
- A NASP (N1/N2)-Related Protein, Sim3, Binds CENP-A and Is Required for Its Deposition at Fission Yeast CentromeresMolecular Cell, 2007
- Histone chaperones: an escort network regulating histone trafficNature Structural & Molecular Biology, 2007
- Chaperone-mediated assembly of centromeric chromatinin vitroProceedings of the National Academy of Sciences of the United States of America, 2006
- The human CENP-A centromeric nucleosome-associated complexNature, 2006