A μ-capture ELISA for detecting Mycoplasma pneumoniae IgM: comparison with indirect immunofluorescence and indirect ELISA
- 1 February 1985
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 94 (2), 217-227
- https://doi.org/10.1017/s0022172400061428
Abstract
SUMMARY: A μ-capture ELISA was developed for detecting Mycoplasma pneumoniae specific IgM, and compared with an indirect immunofluorescent antibody (IFA) technique and an indirect ELISA. μ-capture ELISA and IFA compared well and were found to be the most sensitive assays. The IFA test can be completed in 2 h whilst the results of the μ-capture ELISA can be available in 24 h. Both tests are amenable to routine diagnostic use and have similar sensitivity. Indirect ELISA was found to be less sensitive and less specific, giving high assay values with several sera having undetectable M. pneumoniae CF antibody or CF antibody in low titre. Serum samples obtained from 11 patients at various times after M. pneumoniae infection showed maximum antibody levels within the first month by all assays, with a gradual fall in amount of IgM with time when assayed by μ-capture ELISA, a more gradual decline by IFA and hardly any decline with indirect ELISA. It was concluded that the indirect ELISA is unsuitable for the investigation of possible M. pneumoniae infection because the sustained high assay values with serum samples taken many months after infection, make interpretation of the test results very difficult.This publication has 18 references indexed in Scilit:
- Use of the enzyme-linked immunosorbent assay for the early diagnosis ofMycoplasma pneumoniae infectionEuropean Journal of Clinical Microbiology & Infectious Diseases, 1984
- Antibody assays for varicella‐zoster virus: Comparison of competitive enzyme‐linked immunosorbent assay (ELISA), Competitive radioimmunoassay (RIA), Complement fixation, and indirect immunofluorescence assaysJournal of Medical Virology, 1984
- Rubella-specific IgM reactivity in sera from cases of infectious mononucleosisEpidemiology and Infection, 1983
- A study of the specific IgM antibody response inMycoplasma pneumoniaeinfection in manEpidemiology and Infection, 1983
- Comparison of enzyme-linked immunosorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies.Journal of Clinical Pathology, 1983
- Rubella-specific IgM detected by an antibody capture assay/ELISA technique.Journal of Clinical Pathology, 1981
- MUCOCUTANEOUS REACTIONS DURING MYCOPLASMA PNEUMONIÆ INFECTIONThe Lancet, 1978
- Further Studies on Classification of Bovine Strains of Mycoplasmatales, with Proposals for New Species, Acholeplasma modicum and Mycoplasma alkalescensJournal of General Microbiology, 1973
- IMMUNOCHEMICAL ANALYSIS OF MYCOPLASMA PNEUMONIAE*Annals of the New York Academy of Sciences, 1967
- COLD AGGLUTININS. II. COLD ISOHEMAGGLUTININS IN PRIMARY ATYPICAL PNEUMONIA OF UNKNOWN ETIOLOGY WITH A NOTE ON THE OCCURRENCE OF HEMOLYTIC ANEMIA IN THESE CASESJCI Insight, 1945