Angiotensin II-induced aortic ring constriction is mediated by phosphatidylinositol 3-kinase/L-type calcium channel signaling pathway
Open Access
- 1 January 2009
- journal article
- Published by Springer Science and Business Media LLC in Experimental & Molecular Medicine
- Vol. 41 (8), 569-76
- https://doi.org/10.3858/emm.2009.41.8.062
Abstract
Angiotensin II (AngII) is a crucial hormone that affects vasoconstriction and exerts hypertrophic effects on vascular smooth muscle cells. Here, we showed that phosphatidylinositol 3-kinase-dependent calcium mobilization plays pivotal roles in AngII-induced vascular constriction. Stimulation of rat aortic vascular smooth muscle cell (RASMC)-embedded collagen gel with AngII rapidly induced contraction. AngII-induced collagen gel contraction was blocked by pretreatment with a phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002) whereas ERK inhibitor (PD98059) was not effective. AngII-induced collagen gel contraction was significantly blocked by extracellular calcium depletion by EGTA or by nifedipine which is an L-type calcium channel blocker. In addition, AngII-induced calcium mobilization was also blocked by nifedipine and EGTA, whereas intracellular calcium store-depletion by thapsigargin was not effective. Finally, pretreatment of rat aortic ring with LY294002 and nifedipine significantly reduced AngII-induced constriction. Given these results, we suggest that PI3K-dependent activation of L-type calcium channels might be involved in AngII-induced vascular constriction.Keywords
This publication has 26 references indexed in Scilit:
- Peripheral sympatholytic actions of four AT1 antagonists: are they relevant for long-term antihypertensive efficacy?Journal of Hypertension, 2005
- Regulation of Vascular L-type Ca 2+ Channels by Phosphatidylinositol 3,4,5-TrisphosphateCirculation Research, 2004
- Role of p38 MAPK and MAPKAPK-2 in angiotensin II-induced Akt activation in vascular smooth muscle cellsAmerican Journal of Physiology-Cell Physiology, 2004
- Sevoflurane Inhibits Angiotensin II–induced, Protein Kinase C–mediated but Not Ca2+-elicited Contraction of Rat Aortic Smooth MuscleAnesthesiology, 2004
- Pyk2- and Src-Dependent Tyrosine Phosphorylation of PDK1 Regulates Focal AdhesionsMolecular and Cellular Biology, 2003
- Functional Interplay Between Angiotensin II and Nitric OxideArteriosclerosis, Thrombosis, and Vascular Biology, 2003
- Regulation of Phosphoinositide-Specific Phospholipase CAnnual Review of Biochemistry, 2001
- Novel gp91
phox
Homologues in Vascular Smooth Muscle CellsCirculation Research, 2001
- Temporal Dispersion of Activation of Phospholipase C-β1 and -γ Isoforms by Angiotensin II in Vascular Smooth Muscle CellsOnline Journal of Public Health Informatics, 1998
- Cardiac Actions of Angiotensin II: Role of an Intracardiac Renin-Angiotensin SystemAnnual Review of Physiology, 1992