Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene
Open Access
- 1 October 1986
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 323 (6089), 646-650
- https://doi.org/10.1038/323646a0
Abstract
Duchenne muscular dystrophy (DMD) and the less severe Becker muscular dystrophy (BMD) are human X-linked muscle-wasting disorders that have been localized to the band Xp21 by genetic linkage analysis1–9 and cytologically detectable abnormalities10–12. A cloned DNA segment, DXS164 (or pERT87), has been shown to detect deletions in the DNA of unrelated DMD and BMD males13–15. Here we present the nucleotide sequence of two highly conserved DNA fragments from the DXS164 locus and their homologous sequences from the mouse X chromosome. One of the human conserved segments hybridized to a large transcript in RNA isolated from human fetal skeletal muscle and was used to isolate cDNA clones which cover approximately 10% of this transcript. The cDNA clones map to Xp21 and hybridize with a minimum of eight small regions that span 130 kilobases (kb) of the DXS164 locus. These expressed sequences are candidates for portions of the gene responsible for both DMD and BMD.This publication has 31 references indexed in Scilit:
- RECOMBINATION WITH PERT87 (DXS164) IN FAMILIES WITH X-LINKED MUSCULAR DYSTROPHYThe Lancet, 1986
- Cytogenetic heterogeneity of translocations associated with Duchenne muscular dystrophyClinical Genetics, 1986
- Human Apolipoprotein B: Structure of Carboxyl-Terminal Domains, Sites of Gene Expression, and Chromosomal LocalizationScience, 1985
- PRENATAL DIAGNOSIS AND CARRIER DETECTION OF DUCHENNE MUSCULAR DYSTROPHY WITH CLOSELY LINKED RFLPsThe Lancet, 1985
- Report of the committee on the genetic constitution of the X and Y chromosomesCytogenetic and Genome Research, 1985
- Yeast RNA Polymerase II Genes: Isolation with Antibody ProbesScience, 1983
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975