Aerosolization of Mycoplasma synoviae compared with Mycoplasma gallisepticum and Enterococcus faecalis

Abstract

In order to study the airborne transmission of an arthropathic strain of Mycoplasma synoviae, preliminary aerosol experiments were performed. They were conducted in duplicate in an empty isolator (1.3 m3) to assess the yield and viability of M. synoviae with time compared with Mycoplasma gallisepticum and Enterococcus faecalis. After aerosol generation air samples were taken with two different devices using gelatine or cellulose nitrate filters. There was no difference between the devices, but cellulose nitrate filters yielded very low bacterial counts. The aerosolized dose per isolator for M. synoviae was 3.4 x 10(10) colony-forming units (cfu), for M. gallisepticum was 2.6 x 10(10) cfu and for E. faecalis was 3 x 10(10) cfu. Immediately after aerosolization, concentrations of about 10(6) to 10(7) cfu/m3, 10(7) to 10(8) cfu/m3 and 10(8) to 10(9) cfu/m3 air of M. synoviae, M. gallisepticum and E. faecalis were found, respectively. At 25 min M. synoviae concentrations dropped below the detection level (<4 x 10(4) cfu), while 10(5) to 10(6) and 10(8) to 10(9) cfu were found for M. gallisepticum and E. faecalis, respectively. The average M. synoviae concentration during the experiment was estimated at 10(2) to 10(3) cfu/l. The M. gallisepticum and E. faecalis aerosol generated an average of approximately 10(3) to 10(4) cfu/l air and 10(5) to 10(6) cfu/l air, respectively. Thus mycoplasma and E. faecalis aerosols were successfully generated despite considerable initial loss as measured by culture. The loss was greater in the mycoplasma aerosols, especially those of M. synoviae.