Protective effect of C60‐methionine derivate on lead‐exposed human SH‐SY5Y neuroblastoma cells
- 28 September 2010
- journal article
- research article
- Published by Wiley in Journal of Applied Toxicology
- Vol. 31 (3), 255-261
- https://doi.org/10.1002/jat.1588
Abstract
Oxidative stress has been considered as one of the possible mechanisms leading to the neurotoxicity of lead. One of the effective ways to prevent cellular damage after lead exposure is using antioxidants. In this paper, a novel C60‐methionine derivate (FMD), a fullerene molecule modified with methionine, was synthesized. The protective effect of FMD on lead‐exposed human SH‐SY5Y neuroblastoma cells was investigated. In this research, after incubating with 500 µm Pb acetate alone for 72 h, the cells had undergone a series of biological changes including viability loss, apoptotic death, the depletion of glutathione (GSH), the peroxidation of membrane lipid and DNA damage. Pretreatment with FMD before lead exposure could improve cell survival, increase the GSH level, reduce malondialdehyde content and attenuate DNA damage without obvious toxicity. In addition, the protective effects of FMD were proven to be greater than those of other two C60‐amino acid derivates, β‐alanine C60 derivate and cystine C60 derivate, which have been confirmed in our previous work to be able to protect rat pheochromocytoma PC12 cells from hydrogen dioxide‐induced oxidative injuries. These observations suggest that FMD may serve as a potential antioxidative and neuroprotective agent in the prevention of lead intoxication. Copyright © 2010 John Wiley & Sons, Ltd.Keywords
This publication has 47 references indexed in Scilit:
- Polyhydroxylated fullerene derivative C60(OH)24 prevents mitochondrial dysfunction and oxidative damage in an MPP+‐induced cellular model of Parkinson's diseaseJournal of Neuroscience Research, 2008
- Low level lead exposure and oxidative stress: Current opinionsClinica Chimica Acta; International Journal of Clinical Chemistry, 2007
- Protective effect of 17-β-estradiol in human neurocellular models of lead exposureNeuroToxicology, 2007
- Effects of N-Acetylcysteine on Lead-Exposed PC-12 CellsArchives of Environmental Contamination and Toxicology, 2005
- A biologically effective fullerene (C60) derivative with superoxide dismutase mimetic propertiesFree Radical Biology & Medicine, 2004
- Tea Catechins Protect against Lead-Induced ROS Formation, Mitochondrial Dysfunction, and Calcium Dysregulation in PC12 CellsChemical Research in Toxicology, 2003
- DNA damage in workers exposed to lead using comet assayToxicology, 2003
- Resonant Raman scattering study of undoped fullerene thin filmsSynthetic Metals, 1992
- Evaluation of the Potential Role of Chelation Therapy in Treatment of Low to Moderate Lead ExposuresEnvironmental Health Perspectives, 1990
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976