Contributions to the Optimal Use of Human Blood
- 1 December 1976
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 31 (s1), 55-67
- https://doi.org/10.1111/j.1423-0410.1976.tb02223.x
Abstract
Investigations were performed concerning the influence of the pH on the stability of factor VIII during the collection of blood and during the storage of blood and plasma for varying periods and at varying temperatures. The low pH of ACD [acid citrate dextrose] anticoagulant solution (pH 4.9) caused a loss of factor VIII procoagulant activity of 10-15% during the collection of blood. When less acidic anticoagulant solutions were used, substantial losses of factor VIII occurred during the storage of blood. The optimal pH of both the anticoagulant solution and the stored blood should be between 6.7 and 7.0. No anticoagulant solution is known that meets these requirements. In practice ACD ensures the highest recovery of factor VIII in cryoprecipitate, at least in those cases where the blood donations are stored for several hours before separation and freezing of the plasma.This publication has 6 references indexed in Scilit:
- Contributions to the Optimal Use of Human BloodVox Sanguinis, 1976
- ACTIVATION OF HAGEMAN FACTOR IN SOLID AND FLUID PHASESThe Journal of Experimental Medicine, 1973
- A Comparison of Factor VIII Activity in Cryoprecipitates Prepared from ACD and CPD PlasmaTransfusion, 1972
- Methods for the Production of Clinically Effective Intermediate and High-Purity Factor-VIII ConcentratesBritish Journal of Haematology, 1971
- The Effect of Several Variables on Cryoprecipitated Factor VIII (AHG) ConcentratesTransfusion, 1967
- High-potency Antihæmophilic Factor Concentrate prepared from Cryoglobulin PrecipitateNature, 1964