Antidiabetic, antioxidant, and antilipid peroxidative activities ofDracocephalum polychaetumshoot extract in streptozotocin-induced diabetic rats:In vivoandin vitrostudies
- 7 April 2015
- journal article
- Published by Taylor & Francis Ltd in Pharmaceutical Biology
- Vol. 54 (2), 272-278
- https://doi.org/10.3109/13880209.2015.1033561
Abstract
Dracocephalum polychaetum Bornm. (Lamiaceae) is used in folk medicine and contains antioxidant agents.The objective of this study is to investigate the antidiabetic, antioxidant, and antilipid peroxidative properties of methanol extract of D. polychaetum aerial parts.The effect of extract (200, 300, and 400 mg/kg, b.w.) on oral glucose tolerance test (OGTT) was investigated. Also, extract (300 mg/kg) administered orally in diabetic rats for 14 d then, serum levels of some biochemical factors were evaluated. Pancreas samples were used for the determination of malondealdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase, and catalase enzyme activities. Red blood cells (RBCs) and plasma were used for MDA estimation. Pancreatic α-amylase inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl), and FRAP (ferric reducing antioxidant power) assays were done. The total flavonoid content of the extract was determined by spectrophotometry.Extract (300 mg/kg) decreased serum glucose level (27.1%) significantly at 120 min in OGTT. Serum levels of creatinine, triglycerides, cholesterol, alanine amino transferase and MDA levels in plasma, RBCs, and pancreas significantly decreased in treated (300 mg/kg) diabetic rats, while pancreatic GSH content, superoxide dismutase, and catalase enzymatic activities increased (p < 0.05). The IC50 values for the extract and butyl hydroxyanisole were 5.6 and 1.15 mg/mL in DPPH and 0.155 and 0.062 mg/mL in the FRAP methods, respectively. The extract had no inhibitory effect on α-amylase activity. The total amount of flavonoids of the extract was estimated to be 1.8% (g/g) on the basis of quercetin content.Dracocephalum polychaetum shoot extract has antioxidant, antihyperlipidemic, and antilipid peroxidative properties.Keywords
This publication has 27 references indexed in Scilit:
- Protective effect of apigenin on mouse acute liver injury induced by acetaminophen is associated with increment of hepatic glutathione reductase activityFood & Function, 2013
- Chronic Treatment with Ethanolic Extract of the Leavesof Azadirachta indica Ameliorates Lesions of Pancreatic Islets in Streptozotocin DiabetesInternational Journal of Morphology, 2010
- Inhibition Studies of Bovine Xanthine Oxidase by Luteolin, Silibinin, Quercetin, and CurcuminJournal of Natural Products, 2009
- Green tea attenuates diabetes induced Maillard-type fluorescence and collagen cross-linking in the heart of streptozotocin diabetic ratsPharmacological Research, 2007
- ANTIOXIDANT PROPERTIES OF MOLDAVIAN DRAGONHEAD (DRACOCEPHALUM MOLDAVICA L.)Journal of Food Lipids, 2001
- Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissuesFree Radical Biology & Medicine, 1996
- Effects of cadmium on glutathione peroxidase, superoxide dismutase, and lipid peroxidation in the rat heart: A possible mechanism of cadmium cardiotoxicityToxicology and Applied Pharmacology, 1985
- Superoxide DismutasesPlant Physiology, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- The Autoxidation of Human Red Cell Lipids Induced by Hydrogen PeroxideBritish Journal of Haematology, 1971