Analysis of the complete genomic sequence of an apparently avirulent strain of avian hepatitis E virus (avian HEV) identified major genetic differences compared with the prototype pathogenic strain of avian HEV
- 1 May 2007
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 88 (5), 1538-1544
- https://doi.org/10.1099/vir.0.82754-0
Abstract
Avian hepatitis E virus (HEV) was identified from chickens with hepatitis–splenomegaly syndrome. In this study, the complete genomic sequence of an apparently avirulent strain of avian HEV was determined to be 6649 nt in length, excluding the poly(A) tail, which is 5 nt shorter than the prototype avian HEV. Sequence analyses revealed that the ORF1 has 89.6 % nucleotide sequence identity, with numerous non-silent mutations and deletions, compared to the prototype strain. The ORF2 capsid gene showed 90.7 % sequence identity with six non-silent mutations, and ORF3 had four non-silent mutations with 97 % sequence identity. Overall, the apparently avirulent strain shares only 90.1 % nucleotide sequence identity with the prototype strain. The identification of significant non-silent mutations in the capsid gene and other regions suggests that these mutations may play a role in HEV attenuation. This is the first report of the full-length sequence of an apparently avirulent strain of HEV.Keywords
This publication has 27 references indexed in Scilit:
- A Point Mutation within the Replicase Gene Differentially Affects Coronavirus Genome versus Minigenome ReplicationJournal of Virology, 2005
- Systematic Pathogenesis and Replication of Avian Hepatitis E Virus in Specific-Pathogen-Free Adult ChickensJournal of Virology, 2005
- Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEVJournal of General Virology, 2004
- Consumption of wild boar linked to cases of hepatitis EJournal of Hepatology, 2004
- Genetic identification of avian hepatitis E virus (HEV) from healthy chicken flocks and characterization of the capsid gene of 14 avian HEV isolates from chickens with hepatitis–splenomegaly syndrome in different geographical regions of the United StatesJournal of General Virology, 2004
- Severe Hepatitis E Virus Infection after Ingestion of Uncooked Liver from a Wild BoarThe Journal of Infectious Diseases, 2003
- Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genomeJournal of General Virology, 2003
- Aetiology, clinical course and outcome of sporadic acute viral hepatitis in pregnancy*Journal of Viral Hepatitis, 2003
- Detection by Reverse Transcription-PCR and Genetic Characterization of Field Isolates of Swine Hepatitis E Virus from Pigs in Different Geographic Regions of the United StatesJournal of Clinical Microbiology, 2002
- Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis?Journal of Hepatology, 2000