Inhibition of calcification of glutaraldehyde pretreated porcine aortic valve cusps with sodium dodecyl sulfate: Preincubation and controlled release studies
- 1 December 1993
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 27 (12), 1477-1484
- https://doi.org/10.1002/jbm.820271203
Abstract
Calcification of bioprosthetic heart valves fabricated from glutaraldehyde pretreated bovine pericardium or porcine aortic valves (PAV) is a frequent cause of the failure of these devices. Of all strategies considered thus far, only detergent preincubations using compounds such as sodium dodecyl sulfate (SDS) ingibited PAV bioprosthetic mineralization in circulatory sheep bioprosthetic valve replacements. The present study sought to characterize the mechanism of action of SDS preincubation. Results of transport and material characterization studies showed that SDS had a relatively high affinity for PAV, with a maximum uptake of 167.1 ± 6.8 μg SDS/mg tissue over 24 h at 37°C with a partition coefficient of 19.3. The PAV diffusion of SDS was 1.95 ± 0.35 10−6 cm2/sec. The principal effect of SDS on PAV was phospholipid extraction. The residual organic phospholipid extraction. The residual organic phosphate in the SDS pretreated tissue was 2.22 ± 0.72 nmol/mg tissue compared to the control untreated group with 18.52 ± 2.1 nmol/mg tissue. Incubations of PAV specimens in a 1% SDS solution for 24 h significantly inhibited calcification after 21 days in subdermal implants in 3-week-old male rats (PAV Ca2+ = 18.0 ± 11.8 μg/mg) compared to control (177.8 ± 6.0 μg/mg). In contrast, coimplants of 30% SDS silicone rubber polymers, for regional sustained SDS administration, did not impede PAV calcification in 21 day implants Ca2+ = 166.0 ± 14.0 μg/mg compared to the nondrug silicone matrix controls, (Ca2+ = 173.0 ± 6.6 μg/mg). Thus, we conclude that the mechanisms of SDS inhibition of PAV calcification is due to material effects which occur during preincubation, and is not facilitated by sustained SDS administration. © 1993 John Wiley & Sons, Inc.Keywords
This publication has 26 references indexed in Scilit:
- Effects of metallic ions and diphosphonates on inhibition of pericardial bioprosthetic tissue calcification and associated alkaline phosphatase activityBiomaterials, 1993
- Initiation of mineralization in bioprosthetic heart valves: Studies of alkaline phosphatase activity and its inhibition by AlCl3 or FeCl3 preincubationsJournal of Biomedical Materials Research, 1991
- Prevention of calcification of glutaraldehyde pretreated bovine pericardium through controlled release polymeric implants: studies of Fe3+, Al3+, protamine sulphate and levamisoleBiomaterials, 1990
- Prevention of Calcification of Bioprosthetic heart Valve Leaflets by Ca2+ Diphosphonate PretreatmentJournal of Pharmaceutical Sciences, 1988
- Controlled release of diphosphonate to inhibit bioprosthetic heart valve calcification: Dose-response and mechanistic studiesJournal of Controlled Release, 1986
- Inhibition by diphosphonate compounds of calcification of porcine bioprosthetic heart valve cusps implanted subcutaneously in rats.Circulation, 1985
- Modification by the hancock T6 process of calcification of bioprosthetic cardiac valves implanted in sheepThe American Journal of Cardiology, 1984
- Calcific degeneration as the main cause of porcine bioprosthetic valve failureThe American Journal of Cardiology, 1984
- Calcific deposits in porcine bioprostheses: Structure and pathogenesisThe American Journal of Cardiology, 1980
- The role of synthetic and bone extracted Ca-phospholipid-PO4 complexes in hydroxyapatite formationCalcified Tissue International, 1977