The Phosphorylated Pathway of Serine Biosynthesis Is Essential Both for Male Gametophyte and Embryo Development and for Root Growth in Arabidopsis
Open Access
- 1 June 2013
- journal article
- Published by Oxford University Press (OUP) in THE PLANT CELL ONLINE
- Vol. 25 (6), 2084-2101
- https://doi.org/10.1105/tpc.113.112359
Abstract
This study characterizes the phosphorylated pathway of Ser biosynthesis (PPSB) in Arabidopsis thaliana by targeting phosphoserine phosphatase (PSP1), the last enzyme of the pathway. Lack of PSP1 activity delayed embryo development, leading to aborted embryos that could be classified as early curled cotyledons. The embryo-lethal phenotype of psp1 mutants could be complemented with PSP1 cDNA under the control of Pro35S (Pro35S:PSP1). However, this construct, which was poorly expressed in the anther tapetum, did not complement mutant fertility. Microspore development in psp1.1/psp1.1 Pro35S:PSP1 arrested at the polarized stage. The tapetum from these lines displayed delayed and irregular development. The expression of PSP1 in the tapetum at critical stages of microspore development suggests that PSP1 activity in this cell layer is essential in pollen development. In addition to embryo death and male sterility, conditional psp1 mutants displayed a short-root phenotype, which was reverted in the presence of Ser. A metabolomic study demonstrated that the PPSB plays a crucial role in plant metabolism by affecting glycolysis, the tricarboxylic acid cycle, and the biosynthesis of amino acids. We provide evidence of the crucial role of the PPSB in embryo, pollen, and root development and suggest that this pathway is an important link connecting primary metabolism with development.Keywords
This publication has 52 references indexed in Scilit:
- Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesisNature Genetics, 2011
- PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thalianaThe Plant Journal, 2011
- Arabidopsis plants deficient in plastidial glyceraldehyde-3-phosphate dehydrogenase show alterations in abscisic acid (ABA) signal transduction: interaction between ABA and primary metabolismJournal of Experimental Botany, 2010
- Signaling and Transcriptional Control of Reproductive Development in ArabidopsisCurrent Biology, 2010
- An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data SetsPLOS ONE, 2007
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- ONE-CARBONMETABOLISM INHIGHERPLANTSAnnual Review of Plant Physiology and Plant Molecular Biology, 2001
- Heat-shock tagging: a simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions.The Plant Journal, 2000
- Plastidic Pathway of Serine BiosynthesisJournal of Biological Chemistry, 1999
- Molecular characterization of plastidic phosphoserine aminotransferase in serine biosynthesis fromArabidopsisThe Plant Journal, 1998