HPLC-UV Analysis of Phenol and Chlorophenols in Water After Precolumn Derivatization with 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
- 4 September 2009
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Liquid Chromatography & Related Technologies
- Vol. 32 (16), 2372-2383
- https://doi.org/10.1080/10826070903188013
Abstract
Chlorophenols (2-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,6-dichlorophenol, and 2,4,6-trichlorophenol) are present in drinking water and tap water as a result of disinfection processes employing chlorination. In this study, the levels of phenol and the above chlorophenols in water (100 µL aliquots) were simultaneously analyzed by HPLC-UV (380 nm) after precolumn derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). Standard curves were obtained after derivatization with NBD-F in borate buffer (pH 8.5) at room temperature for 5 min. The six NBD-labeled compounds were well separated in less than 20 min. Plots were linear in the range of 0.01∼0.03 to 0.5 mg/L, with r 2 value ≥0.9953, for all compounds. The lower limits of detection were 0.004 to 0.01 mg/L (signal-to-noise ratio of 3:1). The coefficients of variation were less than 5.7%. The recovery values of phenol and chlorophenols from tap water and natural mineral water spiked with the standard mixture were satisfactory. While the levels of phenol and chlorophenols in water samples tested were below the lower limit of determination, our method is expected to be useful for identifying environmental water samples that are contaminated with phenol and chlorophenols, i.e., for testing whether or not official guidelines are met.Keywords
This publication has 17 references indexed in Scilit:
- Hollow-fiber-supported liquid phase microextraction with in situ derivatization and gas chromatography–mass spectrometry for determination of chlorophenols in human urine samplesJournal of Chromatography B, 2008
- In-column micro-high-performance liquid chromatographic concentration-separation prior to ultraviolet detection for the determination of chlorophenols in water samplesJournal of Chromatography A, 2007
- Direct determination of chlorophenols present in liquid samples by using a supported liquid membrane coupled in-line with capillary electrophoresis equipmentAnalytica Chimica Acta, 2007
- Analysis of phenols in water by high-performance liquid chromatography using coumarin-6-sulfonyl chloride as a fluorogenic precolumn labelJournal of Chromatography A, 2006
- Determination of major phenolic compounds in water by reversed-phase liquid chromatography after pre-column derivatization with benzoyl chlorideJournal of Chromatography A, 2003
- Separation and determination of phenolic compounds by capillary electrophoresis with chemiluminescence detectionJournal of Chromatography A, 2002
- Optimization of a derivatization–solid-phase microextraction method for the analysis of thirty phenolic pollutants in water samplesJournal of Chromatography A, 2002
- Solid-phase microextraction coupled to liquid chromatography for the analysis of phenolic compounds in waterJournal of Chromatography A, 2001
- Determination of pentachlorophenol by flow-injection analysis with spectrophotometric detectionTalanta, 1988
- Study of the behaviour of various phenolic compounds in the 4-aminoantipyrine and ultraviolet-ratio spectrophotometric methods without and with distillationTalanta, 1981