Parietal cell activation by arborization of ECL cell cytoplasmic projections is likely the mechanism for histamine induced secretion of hydrochloric acid
- 23 February 2011
- journal article
- Published by Informa UK Limited in Scandinavian Journal of Gastroenterology
- Vol. 46 (5), 531-537
- https://doi.org/10.3109/00365521.2011.558113
Abstract
Background and aims. Enterochromaffin-like (ECL) cells are central in the regulation of acid secretion. G cells release gastrin and activate ECL cell histamine secretion which stimulates parietal cell H2 receptors initiating acid secretion. It is unclear whether histamine-mediated parietal cell activation is via a vascular or paracrine pathway. To assess this, we utilized immunohistochemistry (IHC) and electron microscopy to examine gastric tissue and used visualization of formalin fixed dispersed gastric cells and glands to investigate and define the anatomical relationship between ECL and parietal cells. Material and methods. Sprague–Dawley rat stomachs were instilled with formalin. Thereafter fixed mucosal cells and whole gastric glands were dispersed by mechanical and chemical dissolution and enzymatic digestion. Smears with fixed isolated cells and whole glands were stained by IHC with histidine decarboxylase (HDC) and H+/K+-ATPase antibodies. Whole tissue samples of Sprague–Dawley and cotton rat oxyntic mucosa were investigated with IHC using HDC, VMAT2 and H+/K+-ATPase antibodies, and electron microscopy was performed to further delineate the precise anatomic relationship between ECL cells and parietal cells. Results. Each ECL cell generated a network of HDC- and VMAT2-positive dendritic-like elongations that were in direct contact with several parietal cells. Thus, ECL cells at the base of the gland were in communication with parietal cells in the middle of the gland. Electron microscopy confirmed that the cytoplasmic ECL cell elongations containing secretory vesicles were in direct juxtaposition to parietal cells. Conclusions. These findings indicate that ECL cells directly regulate parietal cell function in a neurocrine manner via slender neuron-like elongations.Keywords
This publication has 18 references indexed in Scilit:
- Delineation of the Chemomechanosensory Regulation of Gastrin Secretion Using Pure Rodent G CellsGastroenterology, 2009
- Long Slender Cytoplasmic Extensions: A Common Feature of Neuroendocrine Cells?Journal of Neuroendocrinology, 2007
- A new method for visualization of gut mucosal cells, describing the enterochromaffin cell in the rat gastrointestinal tractScandinavian Journal of Gastroenterology, 2006
- ECL cell histamine mobilization and parietal cell stimulation in the rat stomach studied by microdialysis and electron microscopyActa Physiologica, 2005
- Control of secretion from rat stomach ECL cells in situ and in primary cultureScandinavian Journal of Clinical and Laboratory Investigation, 2001
- Histamine-storing cells in the oxyntic mucosa of the rat stomach: a transmission electron microscopic study employing fixation with carbodiimide.Journal of Histochemistry & Cytochemistry, 1993
- Radioimmunoassay of HistamineScandinavian Journal of Gastroenterology, 1991
- Argyrophilic reaction of histamine-containing epithelial cells in murine gastric mucosaCellular and Molecular Life Sciences, 1969
- Mobilization and formation of histamine in the gastric mucosa as related to acid secretionThe Journal of Physiology, 1964
- The chemical mechanism of gastric secretion1The Journal of Physiology, 1906