Genotypic Characterization of Carbapenem-NonsusceptibleAcinetobacterspp. Isolated in Latin America
- 1 December 2004
- journal article
- research article
- Published by Mary Ann Liebert Inc in Microbial Drug Resistance
- Vol. 10 (4), 286-291
- https://doi.org/10.1089/mdr.2004.10.286
Abstract
The principal aim of this study was to evaluate the genomic diversity among the imipenem-nonsusceptible Acinetobacter spp. (INSA) collected from the Latin American medical centers within the SENTRY Antimicrobial Surveillance Program. The INSA isolates were collected from patients with bloodstream infections, who were hospitalized in seven Latin American countries between 1997 and 1999. For epidemiologic comparison, 20 carbapenem-susceptible Acinetobacter spp. (CSA) isolates were collected in the same period of time from the respective medical centers. A total of 23 Acinetobacter spp. isolates exhibiting imipenem MIC values of ≥8 µg/ml were typed by ribotyping, an automated molecular method. The isolates showing an identical ribogroup were also typed by pulsed-field gel electrophoresis (PFGE). The antimicrobial susceptibility to various antimicrobial agents was evaluated using a reference broth microdilution technique. Among the INSA isolates, 13 distinct ribogroups were observed, whereas 16 ribogroups were detected among the CSA. Nearly 57% of the INSA belonged to only four ribogroups. Identical ribogroups and PFGE patterns were observed among INSA and CSA isolates collected from medical centers located in different countries (Brazil and Argentina). Our results showed: (1) a higher genomic variability among the CSA; (2) presence of epidemic clones among INSA isolates encountered in Latin American medical centers; and (3) spread of INSA and CSA epidemic clones between Latin American countries.Keywords
This publication has 26 references indexed in Scilit:
- Epidemiology and Infection Control Implications of Acinetobacter spp. in Hong KongJournal of Clinical Microbiology, 2001
- Nosocomial Bloodstream Infections Caused by Acinetobacter Species in United States Hospitals: Clinical Features, Molecular Epidemiology, and Antimicrobial SusceptibilityClinical Infectious Diseases, 2000
- Activity and spectrum of 22 antimicrobial agents tested against urinary tract infection pathogens in hospitalized patients in Latin America: report from the second year of the SENTRY Antimicrobial Surveillance Program (1998)Journal of Antimicrobial Chemotherapy, 2000
- Genotypic and phenotypic similarity of multiresistant Acinetobacter baumannii isolates in the Czech RepublicJournal of Medical Microbiology, 1999
- Acinetobacter Species as Nosocomial PathogensEuropean Journal of Clinical Microbiology & Infectious Diseases, 1998
- Use of Macrorestriction Analysis to Demonstrate Interhospital Spread of Multiresistant Acinetobacter baumannii in Sao Paulo, BrazilClinical Infectious Diseases, 1996
- An Outbreak of Multiresistant Acinetobacter baumanii in a University Hospital in São Paulo, BrazilInfection Control & Hospital Epidemiology, 1996
- Comparative evaluation of an automated ribotyping system versus pulsed-field gel electrophoresis for epidemiological typing of clinical isolates of Escherichia coli and Pseudomonas aeruginosa from patients with recurrent gram-negative bacteremiaDiagnostic Microbiology and Infectious Disease, 1996
- Molecular Heterogeneity of Acinetobacter baumanii Isolates During Seasonal Increase in PrevalenceInfection Control & Hospital Epidemiology, 1995
- Clinical and molecular epidemiology of acinetobacter infections sensitive only to polymyxin B and sulbactamThe Lancet, 1994