Targeted imaging modality selection for bacterial biofilms in chronic rhinosinusitis
- 30 November 2009
- journal article
- sinonasal
- Published by Wiley in The Laryngoscope
- Vol. 120 (2), 427-431
- https://doi.org/10.1002/lary.20705
Abstract
Objectives/Hypothesis: Biofilms are increasingly recognized as having an etiological role in chronic rhinosinusitis (CRS). Research into biofilms in CRS currently relies on microscopic imaging techniques, none of which are universally accepted. This study compares LIVE/DEAD BacLight (Invitrogen Corp., Carlsbad, CA) staining and fluorescence in situ hybridization (FISH), both utilizing the confocal scanning laser microscope (CSLM) for biofilm determination and characterization in CRS patients. Study Design: Prospective study. Methods: Twenty CRS patients undergoing endoscopic sinus surgery were recruited for the study. Sinus mucosal tissue harvested at the time of surgery underwent both the BacLight/CSLM and FISH/CSLM protocols for biofilm determination and characterization. Results: Combining the results of both protocols, 18/20 (90%) patients had bacterial biofilms demonstrable on at least one modality. The high biofilm detection rate combining the two techniques suggests the prevalence of biofilms in CRS may be greater than previously reported. The protocols had equivalent results in 15/20 patients. Using the differences observed in the remaining five patients, we can highlight the most appropriate use for each technique. Conclusions: BacLight/CSLM and FISH/CSLM are complementary techniques for biofilm determination and characterization. Both protocols are suited to different research areas and the selection of technique used should be based on the specific objectives of the research protocol. In this way we can utilize the advantages of each technique to facilitate effective research. Laryngoscope, 2010Keywords
Funding Information
- Garnett Passe and Rodney Williams Memorial Foundation
This publication has 20 references indexed in Scilit:
- The Effect of Bacterial Biofilms on Post-sinus Surgical OutcomesAmerican Journal of Rhinology, 2008
- Diagnosis and prevention of catheter-related infectionsCurrent Opinion in Critical Care, 2007
- Confocal Scanning Laser Microscopy Evidence of Biofilms in Patients With Chronic RhinosinusitisThe Laryngoscope, 2007
- Bacterial Biofilms on the Sinus Mucosa of Human Subjects With Chronic RhinosinusitisThe Laryngoscope, 2006
- Characterization, Distribution, and Expression of Novel Genes among Eight Clinical Isolates of StreptococcuspneumoniaeInfection and Immunity, 2006
- The Role of Bacteria in Chronic RhinosinusitisOtolaryngologic Clinics of North America, 2005
- Evidence of Bacterial Biofilms in Human Chronic SinusitisORL, 2004
- Editors and Reviewers: Supporting Peer-Review PublishingOtolaryngology -- Head and Neck Surgery, 2003
- Candidate's Thesis: Direct Evidence of Bacterial Biofilms in Otitis MediaThe Laryngoscope, 2001
- Modern microscopy in biofilm research: confocal microscopy and other approachesCurrent Opinion in Biotechnology, 1999