Gas-liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid

Abstract

The theory of the partition column was extended to cover a compressible mobile phase. Satisfactory agreement of expt. with theory was found. Gas-liquid partition columns are described for the separation of volatile fatty acids from formic to dodecanoic. Complete resolution is obtained of all normal acids (and iso acids up to at least C6) on a 4 ft. column and of all the isomers of valeric acid on an 11 ft. column. A method is described for the micro-estimation of volatile fatty acids isolated as their Na salts. A recording burette is described which may be coupled to a photoelectric control circuit to render the analysis automatic.