Coordination of Three Signaling Enzymes by AKAP79, a Mammalian Scaffold Protein
- 15 March 1996
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 271 (5255), 1589-1592
- https://doi.org/10.1126/science.271.5255.1589
Abstract
Multivalent binding proteins, such as the yeast scaffold protein Sterile-5, coordinate the location of kinases by serving as platforms for the assembly of signaling units. Similarly, in mammalian cells the cyclic adenosine 3′,5′-monophosphate-dependent protein kinase (PKA) and phosphatase 2B [calcineurin (CaN)] are complexed by an A kinase anchoring protein, AKAP79. Deletion analysis and binding studies demonstrate that a third enzyme, protein kinase C (PKC), binds AKAP79 at a site distinct from those bound by PKA or CaN. The subcellular distributions of PKC and AKAP79 were similar in neurons. Thus, AKAP79 appears to function as a scaffold protein for three multifunctional enzymes.Keywords
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