Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.
- 1 August 1986
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 83 (16), 5909-5913
- https://doi.org/10.1073/pnas.83.16.5909
Abstract
Transcription from nitrogen-regulated promoters, such as glnAp2, requires the glnG gene product, NRI, as well as the rpoN(glnF) gene product, sigma60, and is regulated by the glnL gene product, NRII. We find that in a reaction mixture containing NRI, NRII, and ATP, NRII catalyzes the transfer of the gamma phosphate of ATP to NRI. This covalent modification of NRI occurs concurrently with the acquisition of the ability by the reaction mixture to activate transcription from glnAp2. In the presence of PII, the product of glnB, NRII catalyzes the removal of the phosphate from NRI-phosphate. This reaction occurs concurrently with the loss by the reaction mixture of the ability to activate transcription from glnAp2. On the basis of this evidence, we propose that NRI-phosphate activates transcription from nitrogen-regulated promoters and that the role of NRII is control of the formation and breakdown of NRI-phosphate in response to cellular signals of nitrogen availability.This publication has 10 references indexed in Scilit:
- Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG, and glnL.Proceedings of the National Academy of Sciences of the United States of America, 1985
- Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.Proceedings of the National Academy of Sciences of the United States of America, 1985
- CELL ADHESION AND THE MOLECULAR PROCESSES OF MORPHOGENESISAnnual Review of Biochemistry, 1985
- Expression of glnA in Escherichia coli is regulated at tandem promoters.Proceedings of the National Academy of Sciences of the United States of America, 1985
- POSITIVE CONTROL OF TRANSCRIPTION INITIATION IN BACTERIAAnnual Review of Genetics, 1984
- Isolation of the nitrogen assimilation regulator NR I , the product of the glnG gene of Escherichia coliProceedings of the National Academy of Sciences of the United States of America, 1983
- Regulation at the glnL-operator-promoter of the complex glnALG operon of Escherichia coli.1983
- A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America, 1979
- The product of a newly identified gene, gInF, is required for synthesis of glutamine synthetase in Salmonella.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Cascade control of Escherichia coli glutamine synthetase. Properties of the PII regulatory protein and the uridylyltransferase-uridylyl-removing enzymeOnline Journal of Public Health Informatics, 1975