Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis inDrosophila
Open Access
- 1 May 2007
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 120 (9), 1689-1700
- https://doi.org/10.1242/jcs.004663
Abstract
In higher organisms, the chromatin of sperm is organised in a highly condensed protamine-based structure. In pre-meiotic stages and shortly after meiosis, histones carry multiple modifications. Here, we focus on post-meiotic stages and show that also after meiosis, histone H3 shows a high overall methylation of K9 and K27 and we hypothesise that these modifications ensure maintenance of transcriptional silencing in the haploid genome. Furthermore, we show that histones are lost during the early canoe stage and that just before this stage, hyper-acetylation of histone H4 and mono-ubiquitylation of histone H2A occurs. We believe that these histone modifications within the histone-based chromatin architecture may lead to better access of enzymes and chromatin remodellers. This notion is supported by the presence of the architectural protein CTCF, numerous DNA breaks, SUMO, UbcD6 and high content of ubiquitin, as well as testes-specific nuclear proteasomes at this time. Moreover, we report the first transition protein-like chromosomal protein, Tpl94D, to be found in Drosophila. We propose that Tpl94D – an HMG box protein – and the numerous DNA breaks facilitate chromatin unwinding as a prelude to protamine and Mst77F deposition. Finally, we show that histone modifications and removal are independent of protamine synthesis.Keywords
This publication has 38 references indexed in Scilit:
- Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesisGenes & Development, 2006
- Changes in intranuclear chromatin architecture induce bipolar nuclear localization of histone variant H1T2 in male haploid spermatidsDevelopmental Biology, 2006
- ING2 PHD domain links histone H3 lysine 4 methylation to active gene repressionNature, 2006
- HANP1/H1T2, a Novel Histone H1-Like Protein Involved in Nuclear Formation and Sperm FertilityMolecular and Cellular Biology, 2005
- CHROMATIN REMODELING IN SPERMATIDS: A SENSITIVE STEP FOR THE GENETIC INTEGRITY OF THE MALE GAMETEArchives of Andrology, 2005
- Loss of HR6B Ubiquitin-Conjugating Activity Results in Damaged Synaptonemal Complex Structure and Increased Crossing-Over Frequency during the Male Meiotic ProphaseMolecular and Cellular Biology, 2003
- Packaging paternal chromosomes with protamineNature Genetics, 2001
- A His2AvDGFP Fusion Gene Complements a Lethal His2AvD Mutant Allele and Provides an in Vivo Marker for Drosophila Chromosome BehaviorDNA and Cell Biology, 1999
- A screen for lethal mutations in the chromosomal region 59AB suggests that bellwether encodes the alpha subunit of the mitochondrial ATP synthase in Drosophila melanogasterMolecular Genetics and Genomics, 1998
- UbcD1, a Drosophila ubiquitin-conjugating enzyme required for proper telomere behavior.Genes & Development, 1997