First report of non-coloured flavonoids inEchium plantagineumbee pollen: differentiation of isomers by liquid chromatography/ion trap mass spectrometry
- 24 February 2010
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 24 (6), 801-806
- https://doi.org/10.1002/rcm.4454
Abstract
6 páginas, 5 figuras, 1 tabla.Apicultural products have been widely used in diet complements as well as in phytotherapy. Bee pollen from Echium plantagineum was analysed by high-performance liquid chromatography/photodiode-array detection coupled to ion trap mass spectrometry (HPLC-PAD-MSn) with an electrospray ionisation interface. The structures have been determined by the study of the ion mass fragmentation, which characterises the interglycosidic linkage in glycosylated flavonoids and differentiates positional isomers. Twelve non-coloured flavonoids were characterised, being kaempferol-3-O-neohesperidoside the major compound, besides others in trace amounts. These include quercetin, kaempferol and isorhamnetin glycosides, with several of them being isomers. Acetylated derivatives are also described. This is the first time that non-coloured flavonoids are reported from this pollen, with MS fragmentation proving to be most useful in the elucidation of isomeric structures.Thanks go to Fundaçäo para a Ciência e a Tecnologia (FCT)\ud for financial support (PTDC/AGR-AAM/64150/2006).\ud D. M. Pereira (BI) is grateful to FCT for the grant.Peer revieweKeywords
Funding Information
- Fundação para a Ciência e a Tecnologia (FCT) (PTDC/AGR-AAM/64150/2006)
- FCT
This publication has 15 references indexed in Scilit:
- New Phenolic Compounds and Antioxidant Potential of Catharanthus roseusJournal of Agricultural and Food Chemistry, 2008
- Hepatotoxic Pyrrolizidine Alkaloids in Pollen and Drying-Related Implications for Commercial Processing of Bee PollenJournal of Agricultural and Food Chemistry, 2008
- HPLC-DAD-MS/MS-ESI Screening of Phenolic Compounds in Pieris brassicae L. Reared on Brassica rapa var.rapaLJournal of Agricultural and Food Chemistry, 2008
- Antioxidative properties of bee pollen in selected plant speciesFood Chemistry, 2007
- Crude protein and amino acid composition of honey bee-collected pollen pellets from south-east Australia and a note on laboratory disparityAustralian Journal of Experimental Agriculture, 2006
- Improved Method for Extraction and LC-MS Analysis of Pyrrolizidine Alkaloids and Their N-Oxides in Honey: Application to Echium vulgare HoneysJournal of Agricultural and Food Chemistry, 2005
- Characterization of the interglycosidic linkage in di‐, tri‐, tetra‐ and pentaglycosylated flavonoids and differentiation of positional isomers by liquid chromatography/electrospray ionization tandem mass spectrometryJournal of Mass Spectrometry, 2004
- Structure characterization of flavonoid O‐diglycosides by positive and negative nano‐electrospray ionization ion trap mass spectrometryJournal of Mass Spectrometry, 2001
- The comparative demography of the pasture weed Echium plantagineum between its native and invaded rangesJournal of Applied Ecology, 2001
- A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugatesGlycoconjugate Journal, 1988