Regeneration of canine peroneal nerve with the use of a polyglycolic acid–collagen tube filled with laminin‐soaked collagen sponge: a comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits
- 1 January 2001
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 58 (6), 622-630
- https://doi.org/10.1002/jbm.1061
Abstract
A novel artificial nerve conduit was developed and its efficiency was evaluated on the basis of promotion of peripheral nerve regeneration across an 80‐mm gap in dogs. The nerve conduit was made of a polyglycolic acid–collagen tube filled with laminin‐soaked collagen sponge. Conduits filled with either sponge‐ or fiber‐form collagen were implanted into an 80‐mm gap of the peroneal nerve (five dogs for each form). Twelve months postoperatively nerve regeneration was superior in the sponge group both morphometrically (percentage of neural tissue: fiber: 39.7 ± 5.2, sponge: 43.0 ± 4.5, n=3) and electrophysiologically (fiber: CMAP 1.06 ± 0.077, SEP 1.32 ± 0.127 sponge: CMAP 1.04 ± 0.106, SEP 1.24 ± 0.197, n=5), although these differences were not statistically significant. The observed regeneration was complementary to successful results reported previously in the same model, in which collagen fibers exclusively were used. The results indicate a possible superiority of collagen sponge over collagen fibers as filling materials. In addition, the mass‐producibility, superior scaffolding potential, and capacity for gradual release of soluble factors of the sponge provide make it an attractive alternative to fine fibers, which are both technologically difficult and costly to produce. This newly developed nerve conduit has the potential to enhance peripheral nerve regeneration across longer gaps commonly encountered in clinical settings. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 58: 622–630, 2001Keywords
This publication has 41 references indexed in Scilit:
- Preparation of Bioabsorbable Nerve Guide TubesArtificial Organs, 2000
- Intrathoracic esophageal replacement in the dog with the use of an artificial esophagus composed of a collagen sponge with a double-layered silicone tubeThe Journal of Thoracic and Cardiovascular Surgery, 1999
- FACIAL NERVE REPAIR USING A COLLAGEN CONDUIT IN CATSScandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, 1999
- Long Acellular Nerve Transplants for Allogeneic Grafting and the Effects of Basic Fibroblast Growth Factor on the Growth of Regenerating Axons in Dogs: A Preliminary ReportExperimental Neurology, 1998
- An immunocytochemical analysis of growing axons in a silicone chamber prefilled with artificial sponge matrixActa Histochemica, 1996
- The Use of Cultured Schwann Cells in Nerve Repair in a Rabbit Hind-Limb ModelJournal of Reconstructive Microsurgery, 1996
- Differential delay of reinnervating axons alters specificity in the rat serratus anterior muscleJournal of Neurobiology, 1995
- Transplantation of a Polymer-Encapsulated Cell Line Genetically Engineered to Release NGFExperimental Neurology, 1993
- Long‐term evaluation of nerve regeneration in a biodegradable nerve guideMicrosurgery, 1993
- Nerve repair using a polyglactin tube and nerve graft: An experimental study in the rabbitBiomaterials, 1983