Recent Advances in the Polymerase Chain Reaction
- 21 June 1991
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 252 (5013), 1643-1651
- https://doi.org/10.1126/science.2047872
Abstract
The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.This publication has 80 references indexed in Scilit:
- Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell modelDevelopmental & Comparative Immunology, 2011
- Detection of Borrelia burgdorferi DNA in Museum Specimens of Ixodes dammini TicksScience, 1990
- Mutations in the p53 gene occur in diverse human tumour typesNature, 1989
- Avoiding false positives with PCRNature, 1989
- Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Diagnosis of Sickle Cell Anemia and β-Thalassemia with Enzymatically Amplified DNA and Nonradioactive Allele-Specific Oligonucleotide ProbesThe New England Journal of Medicine, 1988
- Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune interventionCell, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Rapid Prenatal Diagnosis of Sickle Cell Anemia by a New Method of DNA AnalysisThe New England Journal of Medicine, 1987
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985